#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4322386	B	Agonist activity at human full length LRH1 transfected in human HeLa cells incubated for 24 hrs by renilla luciferase reporter gene assay	Homo sapiens	32		cell-based format	Scientific Literature
2.	ALA4322387	B	Binding affinity to recombinant human LRH1 ligand binding domain (300 to 537 residues) expressed in Escherichia coli BL21 cells assessed as receptor stabilization by measuring change in melting temperature at saturating concentration incubated for overnight at 4 degC by differential scanning fluorimetry relative to RJW100	Homo sapiens	6		cell-based format	Scientific Literature
3.	ALA4322388	B	Agonist activity at recombinant full length SF1 (unknown origin) transfected in human HeLa cells incubated for 24 hrs by renilla luciferase reporter gene assay	Homo sapiens	4		cell-based format	Scientific Literature
4.	ALA4322392	B	Activation of human full length LRH1 T352V mutant transfected in human HeLa cells at 0.3 uM incubated for 24 hrs by renilla luciferase reporter gene assay	Homo sapiens	1		cell-based format	Scientific Literature
5.	ALA4322393	B	Activation of human full length LRH1 M345L mutant transfected in human HeLa cells at 0.3 uM incubated for 24 hrs by renilla luciferase reporter gene assay	Homo sapiens	1		cell-based format	Scientific Literature
6.	ALA4322394	B	Activation of human full length LRH1 H390A mutant transfected in human HeLa cells at 0.3 uM incubated for 24 hrs by renilla luciferase reporter gene assay	Homo sapiens	1		cell-based format	Scientific Literature
7.	ALA4322395	B	Agonist activity at recombinant human LRH1 ligand binding domain (300 to 537 residues) expressed in Escherichia coli BL21 cells assessed as increase in fluorescein-labeled Tif2 peptide coactivator recruitment by fluorescence polarization assay	Homo sapiens	3		cell-based format	Scientific Literature
8.	ALA4322396	B	Agonist activity at human LRH1 expressing in endogenous LRH-1 knockout mouse intestinal enteroids assessed as increase in Cyp11a1 mRNA expression at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Homo sapiens	1		assay format	Scientific Literature
9.	ALA4322397	B	Agonist activity at human LRH1 expressing in endogenous LRH-1 knockout mouse intestinal enteroids assessed as increase in Cyp11b1 mRNA expression at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Homo sapiens	1		assay format	Scientific Literature
10.	ALA4322398	B	Agonist activity at human LRH1 expressing in endogenous LRH-1 knockout mouse intestinal enteroids assessed as increase in IL10 mRNA expression at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Homo sapiens	1		assay format	Scientific Literature
11.	ALA4322399	B	Agonist activity at human LRH1 expressing in endogenous LRH-1 knockout mouse intestinal enteroids assessed as decrease in IL 1-beta mRNA expression at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Homo sapiens	1		assay format	Scientific Literature
12.	ALA4322400	B	Agonist activity at human LRH1 expressing in endogenous LRH-1 knockout mouse intestinal enteroids assessed as decrease in TNF-alpha mRNA expression at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Homo sapiens	1		assay format	Scientific Literature
13.	ALA4322401	B	Agonist activity at human full length LRH1 transfected in human HeLa cells assessed as relative efficacy incubated for 24 hrs by renilla luciferase reporter gene assay relative to RJW100	Homo sapiens	15		cell-based format	Scientific Literature
14.	ALA4322403	B	Binding affinity to recombinant human LRH1 ligand binding domain (300 to 537 residues) expressed in Escherichia coli BL21 cells assessed as destabilization of N-terminal portion of helix 7 at AFB region of receptor at 5-fold molar excess to protein concentration incubated overnight at 4 degC by HDX mass spectrometry	Homo sapiens	1		cell-based format	Scientific Literature
15.	ALA4322404	B	Binding affinity to recombinant human LRH1 ligand binding domain (300 to 537 residues) expressed in Escherichia coli BL21 cells assessed as stabilization of loop between helices 6 and 7 at AFB region of receptor at 5-fold molar excess to protein concentration incubated overnight at 4 degC by HDX mass spectrometry	Homo sapiens	1		cell-based format	Scientific Literature
16.	ALA4322407	F	Effect on Cyp11a1 mRNA expression in LRH1 knock-out mouse enteroids at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Mus musculus	1		assay format	Scientific Literature
17.	ALA4322408	F	Effect on Cyp11b1 mRNA expression in LRH1 knock-out mouse enteroids at 1 uM pretreated with TNF-alpha followed incubation with compound for overnight by real-time PCR analysis	Mus musculus	1		assay format	Scientific Literature
18.	ALA4625167	B	Agonist activity at LRH1 LBD (unknown origin) assessed as reduction in NCOR1 recruitment by microarray assay	Homo sapiens	2		single protein format	Scientific Literature
19.	ALA4625168	B	Agonist activity at LRH1 LBD (unknown origin) assessed as reduction in NCOR2 recruitment by microarray assay	Homo sapiens	2		single protein format	Scientific Literature
20.	ALA4625169	B	Agonist activity at LRH1 LBD (unknown origin) assessed as increase in NCOA1 recruitment by microarray assay	Homo sapiens	2		single protein format	Scientific Literature