# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4352728 | B | Inhibition of Plasmodium falciparum N-terminal His6-tagged falcipain-2 (35 residues) expressed in Escherichia coli M15 pREP4 using Z-Lue-Arg-AMC as substrate measured after 30 mins by spectrofluorometric method | Plasmodium falciparum | 20 | ALA4350969 | assay format | Scientific Literature | |
2. | ALA4352729 | B | Inhibition of Plasmodium falciparum N-terminal His-tagged falcipain-3 (33 residues) expressed in Escherichia coli M15 pREP4 using Z-Leu-Arg-AMC as substrate measured after 30 mins by spectrofluorometric method | Plasmodium falciparum | 20 | ALA4350969 | assay format | Scientific Literature | |
3. | ALA4352730 | F | Antimalarial activity against synchronized ring stage CQ-sensitive Plasmodium falciparum 3D7 infected in human erythrocytes incubated for 24 hrs followed by compound washout and subsequent compound addition and measured after 48 hrs by YOYO-1 probe-based flow cytometry | Plasmodium falciparum | 20 | ALA4350969 | organism-based format | Scientific Literature | |
4. | ALA4352731 | F | Antimalarial activity against synchronized ring stage CQ-resistant Plasmodium falciparum W2 infected in human erythrocytes incubated for 24 hrs followed by compound washout and subsequent compound addition and measured after 48 hrs by YOYO-1 probe-based flow cytometry | Plasmodium falciparum | 20 | ALA4350969 | organism-based format | Scientific Literature | |
5. | ALA4352732 | A | Half life in Swiss mouse plasma at 1 ug/ml by UPLC-MS analysis | Mus musculus | 6 | ALA4350969 | cell-free format | Scientific Literature | |
6. | ALA4352733 | A | Half life in Swiss mouse blood at 1 ug/ml by UPLC-MS analysis | Mus musculus | 6 | ALA4350969 | tissue-based format | Scientific Literature | |
7. | ALA4352735 | A | Cytotoxicity against HEK293 cells assessed as reduction in cell viability at 25 uM measured after 18 hrs by alamar blue assay | Homo sapiens | 6 | ALA4350969 | cell-based format | Scientific Literature | |
8. | ALA4352737 | A | Drug metabolism in human liver microsomes assessed as N,N-bis-demethylation at 10 uM measured after 60 mins in presence of NADPH-generating system by UPLC-MS analysis | Homo sapiens | 3 | ALA4350969 | microsome format | Scientific Literature | |
9. | ALA4352739 | A | Drug metabolism in mouse liver microsomes assessed as N,N-bis-demethylation at 10 uM measured after 60 mins in presence of NADPH-generating system by UPLC-MS analysis | Mus musculus | 3 | ALA4350969 | microsome format | Scientific Literature | |
10. | ALA4352740 | A | Drug metabolism in human liver microsomes assessed as GSH-adduct formation at 10 uM measured after 60 mins in presence of NADPH-generating system/GSH-EE by UPLC-MS analysis | Homo sapiens | 6 | ALA4350969 | microsome format | Scientific Literature | |
11. | ALA4352741 | A | Drug metabolism in mouse liver microsomes assessed as GSH-adduct formation at 10 uM measured after 60 mins in presence of NADPH-generating system/GSH-EE by UPLC-MS analysis | Mus musculus | 6 | ALA4350969 | microsome format | Scientific Literature |