# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4316545 | B | Inhibition of His-tagged JMJD6 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as increase in H4 arginine 3 symmetrical dimethylation (H4R3me2s) content at 10 uM using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr by immunoblotting analysis | Homo sapiens | 5 | ALA4312042 | assay format | Scientific Literature | |
2. | ALA4316546 | B | Inhibition of JMJD6 in human HeLa cells assessed as decrease in cell proliferation measured after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay | Homo sapiens | 1 | ALA4312042 | cell-based format | Scientific Literature | |
3. | ALA4316547 | B | Inhibition of JMJD6 in human SMCC7721 cells assessed as decrease in cell proliferation measured after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay | Homo sapiens | 1 | ALA4312042 | cell-based format | Scientific Literature | |
4. | ALA4316548 | B | Inhibition of His-tagged JMJD6 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as decrease in demethylase activity by measuring release of formaldehyde using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
5. | ALA4316549 | B | Inhibition of JMJD6 in human HeLa cells assessed as increase in HSP70R469me1 level at 10 uM incubated for 6 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4312042 | cell-based format | Scientific Literature | |
6. | ALA4316550 | B | Inhibition of JMJD6 in human HeLa cells assessed as H4R3me2S level at 10 uM incubated for 6 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4312042 | cell-based format | Scientific Literature | |
7. | ALA4316551 | A | Cytotoxicity in human LO2 cells assessed as reduction in cell proliferation incubated for 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay | Homo sapiens | 1 | ALA4312042 | cell-based format | Scientific Literature | |
8. | ALA4316552 | B | Inhibition of Flag-tagged PHF8 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as formaldehyde release using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
9. | ALA4316553 | B | Inhibition of Flag-tagged KDM3A (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as formaldehyde release using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
10. | ALA4316554 | B | Inhibition of Flag-tagged KDM4C (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as formaldehyde release using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
11. | ALA4316555 | B | Inhibition of Flag-tagged KDM5C (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as formaldehyde release using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
12. | ALA4316556 | B | Binding affinity to immobilized His-tagged JMJD6 (unknown origin) expressed in Escherichia coli BL21 (DE3) by biacore-binding assay | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature |