# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4397534 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 12.5 to 200 nM at 10 to 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
2. | ALA4397535 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 10 to 25 degreeC by surface plasmon resonance analysis relative to control | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
3. | ALA4397536 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 10 to 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
4. | ALA4397537 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
5. | ALA4397538 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
6. | ALA4397539 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
7. | ALA4397543 | B | Inhibition of Halo-tagged biotinylated human eIF4E expressed in Escherichia coli/Halo-tagged methyltetrazine-labelled human 4E-BP1 expressed in Escherichia coli protein-protein interaction incubated for 1 hr by catalytic enzyme-linked click chemistry assay | Homo sapiens | 7 | assay format | Scientific Literature | ||
8. | ALA4397552 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 25 degreeC in presence of 1000 mM of NaCl by surface plasmon resonance analysis | Homo sapiens | 2 | cell-based format | Scientific Literature |