# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4432347 | Physicochemical | Aqueous solubility of the compound in PBS assessed as precipitate formation at pH 7 after 48 hrs | 11 | ALA4431340 | small-molecule physicochemical format | Scientific Literature | ||
2. | ALA4432348 | Physicochemical | Aqueous solubility of the compound in PBS at 1 to 10 mg/ml assessed as precipitate formation at pH 7 at 4 degC after 7 days | 4 | ALA4431340 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA4432349 | Functional | Activation of glucagon receptor (unknown origin) transfected in HEK293 cells coinfected with luciferase reporter gene linked CRE element assessed as cAMP accumulation after 5 hrs in presence of luclite substrate by liquid scintillation counting | Homo sapiens | 11 | ALA4431340 | cell-based format | Scientific Literature | |
4. | ALA4432350 | Functional | Activation of glucagon receptor (unknown origin) transfected in HEK293 cells co-transfected with luciferase reporter gene linked CRE element assessed as compound EC50 preincubated in PBS buffer for 4 weeks at elevated temperature and pH 7 followed by protein addition measured after 5 hrs in presence of luclite substrate by liquid scintillation counting relative to control | Homo sapiens | 1 | ALA4431340 | cell-based format | Scientific Literature | |
5. | ALA4432351 | Unassigned | Fibrillation of the compound in aqueous sodium phosphate buffer at pH 7.4 assessed as glucagon bioactivity at 5 mg/ml preincubated for 48 hrs without agitation followed by further incubation for 48 hrs with agitation by thioflavin-T fluorescence spectroscopic assay | 10 | ALA4431340 | assay format | Scientific Literature | ||
6. | ALA4432352 | Functional | Antihypoglycemic activity in lean Wistar rat assessed as prevention of insulin-induced glucose reduction at 15 nmol/kg, ip after 60 mins by glucometer | Rattus norvegicus | 2 | ALA4431340 | organism-based format | Scientific Literature | |
7. | ALA4432353 | Functional | Antihypoglycemic activity in lean Wistar rat assessed as prevention of insulin-induced glucose reduction at 15 nmol/kg, ip after 30 mins by glucometer | Rattus norvegicus | 2 | ALA4431340 | organism-based format | Scientific Literature | |
8. | ALA4432354 | Functional | Hyperglycemic activity in lean Wistar rat assessed as elevation of plasma glucose levels at 10 nmol/kg, ip after 120 mins in absence of insulin by glucometer | Rattus norvegicus | 2 | ALA4431340 | organism-based format | Scientific Literature | |
9. | ALA4432355 | Functional | Antihypoglycemic activity in diabetic Yucatan pig assessed as prevention of insulin detemir-induced blood glucose reduction at 10 nmol/kg, sc administered over 60 mins post insulin detemir challenge measured during compound dosing by glucometer | Sus scrofa | 2 | ALA4431340 | organism-based format | Scientific Literature |