# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4340697 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as succinate production at 500 uM incubated for 60 s in presence of 2OG and L-ascorbate and using Fe2 as cofactor by 1H NMR spectroscopy | Paramecium bursaria Chlorella virus 1 | 2 | ALA4340506 | cell-based format | Scientific Literature | |
2. | ALA4340698 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as succinate production at 500 uM incubated for 60 s in presence of 2OG and in absence of L-ascorbate and using Fe2 as cofactor by 1H NMR spectroscopy | Paramecium bursaria Chlorella virus 1 | 2 | ALA4340506 | cell-based format | Scientific Literature | |
3. | ALA4340700 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate Kcat incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | ALA4340506 | cell-based format | Scientific Literature | |
4. | ALA4340701 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate Km incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | ALA4340506 | cell-based format | Scientific Literature | |
5. | ALA4340705 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as Km(app) for Fe2 incubated for 16 hrs in presence of 2OG and Fe2 by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 2 | ALA4340506 | cell-based format | Scientific Literature | |
6. | ALA4340706 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as Km for 2OG incubated for 16 hrs in presence of 2OG and Fe2 by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 2 | ALA4340506 | cell-based format | Scientific Literature | |
7. | ALA4340713 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as Km(app) for ascorbate incubated for 16 hrs in presence of ascorbate, 2OG and Fe2 by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 2 | ALA4340506 | cell-based format | Scientific Literature |