#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4324546	B	Inhibition of recombinant full length human His-tagged MEK1 expressed in baculovirus expression system using ERK K52R dead variant as substrate preincubated for 20 mins followed by incubation with 33P-ATP addition for 2 hrs by hotspot kinase assay	Homo sapiens	3	ALA4321854	assay format	Scientific Literature
2.	ALA4324547	B	Inhibition of recombinant full length human His-tagged MEK2 expressed in baculovirus expression system using ERK K52R dead variant as substrate preincubated for 20 mins followed by incubation with 33P-ATP for 2 hrs by hotspot kinase assay	Homo sapiens	3	ALA4321854	assay format	Scientific Literature
3.	ALA4324580	B	Protac activity against VHL/MEK1 in human HT-29 cells harboring with BRAF V600E variant assessed as degradation of MEK1 by measuring MEK1 protein level up to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
4.	ALA4324581	B	Protac activity against VHL/MEK2 in human HT-29 cells harboring with BRAF V600E variant assessed as degradation of MEK2 by measuring MEK2 protein level up to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
5.	ALA4324582	B	Protac activity against VHL/MEK1 in human SK-MEL-28 cells harboring with BRAF V600E variant assessed as degradation of MEK1 by measuring MEK1 protein level up to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
6.	ALA4324583	B	Protac activity against VHL/MEK2 in human SK-MEL-28 cells harboring with BRAF V600E variant assessed as degradation of MEK2 by measuring MEK2 protein level up to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
7.	ALA4324584	B	Inhibition of MEK1/2 in human HT-29 cells assessed as reduction in phosphorylation of ERK at T202/Y204 residues at 0.1 to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
8.	ALA4324585	B	Inhibition of MEK1/2 in human HT-29 cells assessed as reduction in phosphorylation of MEK at S218/222 residues at 0.1 to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
9.	ALA4324586	B	Inhibition of MEK1/2 in human SK-MEL-28 cells assessed as reduction in phosphorylation of ERK at T202/Y204 residues at 0.1 to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
10.	ALA4324587	B	Inhibition of MEK1/2 in human SK-MEL-28 cells assessed as reduction in phosphorylation of MEK at S218/222 residues at 0.1 to 1 uM after 24 hrs by Western blot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
11.	ALA4324642	B	Protac activity against VHL/MEK1 in human HT-29 cells harboring with BRAF V600E variant assessed as degradation of MEK1 by measuring reduction in MEK1 protein level at 0.1 uM after 10 hrs by LC-MS based Volcano plot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
12.	ALA4324643	B	Protac activity against VHL/MEK2 in human HT-29 cells harboring with BRAF V600E variant assessed as degradation of MEK2 by measuring reduction in MEK2 protein level at 0.1 uM after 10 hrs by LC-MS based Volcano plot analysis	Homo sapiens	2	ALA4321854	cell-based format	Scientific Literature
13.	ALA4324648	F	Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth after 3 days by MTT assay	Homo sapiens	3	ALA4321854	cell-based format	Scientific Literature
14.	ALA4324649	F	Antiproliferative activity against human SK-MEL-28 cells assessed as reduction in cell growth after 3 days by MTT assay	Homo sapiens	3	ALA4321854	cell-based format	Scientific Literature
15.	ALA4324650	F	Antiproliferative activity against human COLO205 cells assessed as reduction in cell growth after 3 days by WST8 assay	Homo sapiens	3	ALA4321854	cell-based format	Scientific Literature
16.	ALA4324651	F	Antiproliferative activity against human UACC257 cells assessed as reduction in cell growth after 3 days by MTT assay	Homo sapiens	3	ALA4321854	cell-based format	Scientific Literature
17.	ALA4324652	F	Inhibition of colony formation in human HT-29 cells at 10 to 100 nM supplemented with fresh medium containing compound every 3 days for 14 days by crystal violet staining based assay	Homo sapiens	3	ALA4321854	cell-based format	Scientific Literature
18.	ALA4324653	F	Inhibition of colony formation in human SK-MEL-28 cells at 10 to 100 nM supplemented with fresh medium containing compound every 3 days for 14 days by crystal violet staining based assay	Homo sapiens	3	ALA4321854	cell-based format	Scientific Literature