#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4397537	B	Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 25 degreeC by surface plasmon resonance analysis	Homo sapiens	7		cell-based format	Scientific Literature
2.	ALA4397538	B	Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 25 degreeC by surface plasmon resonance analysis	Homo sapiens	6		cell-based format	Scientific Literature
3.	ALA4397539	B	Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 25 degreeC by surface plasmon resonance analysis	Homo sapiens	6		cell-based format	Scientific Literature
4.	ALA4397543	B	Inhibition of Halo-tagged biotinylated human eIF4E expressed in Escherichia coli/Halo-tagged methyltetrazine-labelled human 4E-BP1 expressed in Escherichia coli protein-protein interaction incubated for 1 hr by catalytic enzyme-linked click chemistry assay	Homo sapiens	7		assay format	Scientific Literature
5.	ALA4397544	A	Permeability in human MDA-MB-231 cells at 1 uM after 5 hrs in presence of 10% FBS using FITC-labelled compound by Hoechst 33342 staining-based laser confocal microscopy	Homo sapiens	3		cell-based format	Scientific Literature
6.	ALA4397545	B	Inhibition of eIF4E/4E-BP1 interaction in human MDA-MB-231 cells incubated for 6 hr by m7GDP pull down assay	Homo sapiens	2		cell-based format	Scientific Literature
7.	ALA4397546	B	Inhibition of eIF4E/eIF4G interaction in human MDA-MB-231 cells incubated for 6 hr by m7GDP pull down assay	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA4397547	A	Effect on total eIF4E level in human MDA-MB-231 cells at 5 uM after 6 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
9.	ALA4397548	A	Effect on total 4E-BP1 level in human MDA-MB-231 cells at 5 uM after 6 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA4397549	A	Effect on total eIF4G level in human MDA-MB-231 cells at 5 uM after 6 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
11.	ALA4397550	F	Anti-proliferative activity against human MDA-MB-231 cells at 5 to 20 uM after 6 days by CellTiter-Glo assay	Homo sapiens	3		cell-based format	Scientific Literature
12.	ALA4397551	A	Induction of cell lysis in human MDA-MB-231 cells assessed as LDH leakage at 15 uM after 16 hrs by LDH cytotoxicity assay	Homo sapiens	1		cell-based format	Scientific Literature