# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4397537 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 7 | ALA4396874 | cell-based format | Scientific Literature | |
2. | ALA4397538 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 6 | ALA4396874 | cell-based format | Scientific Literature | |
3. | ALA4397539 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 6 | ALA4396874 | cell-based format | Scientific Literature | |
4. | ALA4397543 | B | Inhibition of Halo-tagged biotinylated human eIF4E expressed in Escherichia coli/Halo-tagged methyltetrazine-labelled human 4E-BP1 expressed in Escherichia coli protein-protein interaction incubated for 1 hr by catalytic enzyme-linked click chemistry assay | Homo sapiens | 7 | ALA4396874 | assay format | Scientific Literature | |
5. | ALA4397544 | A | Permeability in human MDA-MB-231 cells at 1 uM after 5 hrs in presence of 10% FBS using FITC-labelled compound by Hoechst 33342 staining-based laser confocal microscopy | Homo sapiens | 3 | ALA4396874 | cell-based format | Scientific Literature | |
6. | ALA4397545 | B | Inhibition of eIF4E/4E-BP1 interaction in human MDA-MB-231 cells incubated for 6 hr by m7GDP pull down assay | Homo sapiens | 2 | ALA4396874 | cell-based format | Scientific Literature | |
7. | ALA4397546 | B | Inhibition of eIF4E/eIF4G interaction in human MDA-MB-231 cells incubated for 6 hr by m7GDP pull down assay | Homo sapiens | 2 | ALA4396874 | cell-based format | Scientific Literature | |
8. | ALA4397547 | A | Effect on total eIF4E level in human MDA-MB-231 cells at 5 uM after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA4396874 | cell-based format | Scientific Literature | |
9. | ALA4397548 | A | Effect on total 4E-BP1 level in human MDA-MB-231 cells at 5 uM after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA4396874 | cell-based format | Scientific Literature | |
10. | ALA4397549 | A | Effect on total eIF4G level in human MDA-MB-231 cells at 5 uM after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA4396874 | cell-based format | Scientific Literature | |
11. | ALA4397550 | F | Anti-proliferative activity against human MDA-MB-231 cells at 5 to 20 uM after 6 days by CellTiter-Glo assay | Homo sapiens | 3 | ALA4396874 | cell-based format | Scientific Literature | |
12. | ALA4397551 | A | Induction of cell lysis in human MDA-MB-231 cells assessed as LDH leakage at 15 uM after 16 hrs by LDH cytotoxicity assay | Homo sapiens | 1 | ALA4396874 | cell-based format | Scientific Literature |