#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4407308	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric affinity cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
2.	ALA4407309	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring efficacy cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
3.	ALA4407310	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric binding affinity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
4.	ALA4407311	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric index in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	67	ALA4406806	cell-based format	Scientific Literature
5.	ALA4407312	Functional	Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	5	ALA4406806	cell-based format	Scientific Literature
6.	ALA4407313	Functional	Positive allosteric modulation of human GPR4 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production at higher concentration in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	4	ALA4406806	cell-based format	Scientific Literature
7.	ALA4407314	Functional	Positive allosteric modulation of human GPR65 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production at higher concentration in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Homo sapiens	4	ALA4406806	cell-based format	Scientific Literature
8.	ALA4407315	Binding	Displacement of [3H]LSD from recombinant human 5HT2B stably expressed in HEK cell membranes incubated for 90 mins under dark condition by microbeta scintillation counting analysis	Homo sapiens	3	ALA4406806	cell-based format	Scientific Literature
9.	ALA4407317	Functional	Agonist activity at human 5HT2B receptor expressed in Flp-In-HEK cells co-expressing Gq assessed as stimulation of calcium mobilization measured for 2 mins by fluo-4 calcium dye-based FLIPR assay	Homo sapiens	3	ALA4406806	cell-based format	Scientific Literature
10.	ALA4407318	Functional	Antagonist activity at human 5HT2B receptor expressed in Flp-In-HEK cells co-expressing Gq assessed as inhibition of 5HT-induced stimulation of calcium mobilization preincubated for 10 mins followed by 5HT addition by fluo-4 calcium dye-based FLIPR assay	Homo sapiens	3	ALA4406806	cell-based format	Scientific Literature
11.	ALA4407322	Binding	Agonist activity at human MT1 receptor expressed in HTLA cells assessed as stimulation of beta-arrestin recruitment at 1 uM incubated for 16 to 20 hrs by bright-glo luminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
12.	ALA4407323	Binding	Agonist activity at human MT2 receptor expressed in HTLA cells assessed as stimulation of beta-arrestin recruitment at 1 uM incubated for 16 to 20 hrs by bright-glo luminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
13.	ALA4407325	Binding	Agonist activity at human MT1 receptor expressed in HTLA cells assessed as stimulation of beta-arrestin recruitment incubated for 16 to 20 hrs by bright-glo luminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
14.	ALA4407326	Binding	Agonist activity at human MT2 receptor expressed in HTLA cells assessed as stimulation of beta-arrestin recruitment incubated for 16 to 20 hrs by bright-glo luminescence assay	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
15.	ALA4407327	Binding	Agonist activity at human MT2 receptor expressed in HTLA cells assessed as stimulation of beta-arrestin recruitment incubated for 16 to 20 hrs by bright-glo luminescence assay relative to control	Homo sapiens	1	ALA4406806	cell-based format	Scientific Literature
16.	ALA4407329	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric affinity cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
17.	ALA4407330	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring efficacy cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
18.	ALA4407331	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric binding affinity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
19.	ALA4407332	Functional	Positive allosteric modulation of mouse GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric index in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay	Mus musculus	6	ALA4406806	cell-based format	Scientific Literature
20.	ALA4407333	ADME	Drug uptake in Swiss albino mouse plasma at 25 mg/kg, ip administered as single dose measured after 0.5 hrs	Mus musculus	1	ALA4406806	organism-based format	Scientific Literature