# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4339580 | B | Inhibition of C-terminal fluorescein-labeled human TCF4 (7 to 51 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 measured after 2.5 hrs by fluorescence polarization assay | Homo sapiens | 58 | ALA4339194 | assay format | Scientific Literature | |
2. | ALA4339581 | B | Inhibition of C-terminal fluorescein-labeled human cadherin (819 to 873 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 measured after 2.5 hrs by fluorescence polarization assay | Homo sapiens | 2 | ALA4339194 | assay format | Scientific Literature | |
3. | ALA4339582 | B | Inhibition of C-terminal fluorescein-labeled human APC-R3 (1477 to 1519 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 measured after 2.5 hrs by fluorescence polarization assay | Homo sapiens | 2 | ALA4339194 | assay format | Scientific Literature | |
4. | ALA4339583 | B | Selectivity ratio of Ki for inhibition of C-terminal fluorescein-labeled human cadherin (819 to 873 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 to Ki for Inhibition of C-terminal fluorescein-labeled human TCF4 (7 to 51 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 | Homo sapiens | 2 | ALA4339194 | assay format | Scientific Literature | |
5. | ALA4339584 | B | Selectivity ratio of Ki for inhibition of C-terminal fluorescein-labeled human APC-R3 (1477 to 1519 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 to Ki for Inhibition of C-terminal fluorescein-labeled human TCF4 (7 to 51 residues) binding to wild-type C-terminal His6-tagged beta-catenin (138 to 781 residues) expressed in Escherichia coli BL21 DE3 | Homo sapiens | 2 | ALA4339194 | assay format | Scientific Literature | |
6. | ALA4339585 | F | Cytotoxicity against human SW480 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay | Homo sapiens | 4 | ALA4339194 | cell-based format | Scientific Literature | |
7. | ALA4339586 | F | Cytotoxicity against human HCT116 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay | Homo sapiens | 4 | ALA4339194 | cell-based format | Scientific Literature | |
8. | ALA4339587 | F | Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay | Homo sapiens | 4 | ALA4339194 | cell-based format | Scientific Literature | |
9. | ALA4339588 | F | Cytotoxicity against human MDA-MB-468 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay | Homo sapiens | 4 | ALA4339194 | cell-based format | Scientific Literature | |
10. | ALA4339589 | F | Cytotoxicity against human BT20 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay | Homo sapiens | 4 | ALA4339194 | cell-based format | Scientific Literature | |
11. | ALA4339590 | F | Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay | Homo sapiens | 4 | ALA4339194 | cell-based format | Scientific Literature | |
12. | ALA4339594 | B | Inhibition of TCF4 binding to beta-catenin in human SW480 cell lysates measured after 4 hrs by co-immunoprecipitation assay | Homo sapiens | 1 | ALA4339194 | subcellular format | Scientific Literature | |
13. | ALA4339611 | B | Inhibition of beta-catenin signaling (unknown origin) expressed in HEK293 cells measured after 24 hrs by dual-glo TOPFlash reporter gene assay | Homo sapiens | 2 | ALA4339194 | cell-based format | Scientific Literature | |
14. | ALA4339619 | A | Drug uptake in human SW480 cells assessed as cell-bound level per million cells at 25 uM measured after 3 hrs by HPLC-MS analysis | Homo sapiens | 2 | ALA4339194 | cell-based format | Scientific Literature | |
15. | ALA5124445 | B | Binding affinity to beta-catenin/Tcf (unknown origin) assessed as inhibition constant | Homo sapiens | 2 | ALA5120880 | protein format | Scientific Literature | |
16. | ALA5124446 | B | Binding affinity to beta-catenin/E-cadherin (unknown origin) assessed as inhibition constant | Homo sapiens | 2 | ALA5120880 | protein format | Scientific Literature | |
17. | ALA5124447 | B | Binding affinity to beta-catenin/APC (unknown origin) assessed as inhibition constant | Homo sapiens | 2 | ALA5120880 | assay format | Scientific Literature | |
18. | ALA5124448 | B | Selectivity ratio of Ki for Tcf4 (unknown origin) to Ki for E-cadherin (unknown origin) | Homo sapiens | 2 | ALA5120880 | assay format | Scientific Literature | |
19. | ALA5124449 | B | Selectivity ratio of Ki for Tcf4 (unknown origin) to Ki for APC (unknown origin) | Homo sapiens | 2 | ALA5120880 | assay format | Scientific Literature | |
20. | ALA5236762 | F | Antiproliferative activity against human SW480 cells assessed as inhibition of cell proliferation incubated for 24 hrs by MTT assay | Homo sapiens | 2 | ALA5236544 | cell-based format | Scientific Literature |