| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4357633 | B | Inhibition of recombinant human SphK1 transfected in HEK293T cells at 1 uM using D-erythro-sphingosine as substrate incubated for 20 mins in presence of [gamma32P]-ATP by liquid scintillation counting method relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 2. | ALA4357634 | B | Inhibition of recombinant human SphK2 transfected in HEK293T cells at 1 uM using D-erythro-sphingosine as substrate incubated for 20 mins in presence of [gamma32P]-ATP by liquid scintillation counting method relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 3. | ALA4357635 | B | Inhibition of recombinant human SphK2 transfected in HEK293T cells at 0.3 uM using D-erythro-sphingosine as substrate incubated for 20 mins in presence of [gamma32P]-ATP by liquid scintillation counting method relative to control | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 4. | ALA4357636 | B | Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using varying level of D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation proximity assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 5. | ALA4357637 | B | Inhibition of human SphK1 expressed in Saccharomyces cerevisiae KYA1 assessed as yeast growth measured after 24 hrs | Homo sapiens | 7 | assay format | Scientific Literature | ||
| 6. | ALA4357638 | B | Inhibition of human SphK2 expressed in Saccharomyces cerevisiae KYA1 assessed as yeast growth measured after 24 hrs | Homo sapiens | 7 | assay format | Scientific Literature | ||
| 7. | ALA4357639 | B | Inhibition of recombinant human SphK2 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]S1P production using varying level of D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation proximity assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 8. | ALA4357640 | B | Selectivity index, ratio of Ki for recombinant human SphK1 expressed in baculovirus infected Sf9 cells to Ki for recombinant human SphK2 expressed in baculovirus infected Sf9 cells using varying level of D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation proximity assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 9. | ALA4357641 | F | In vivo inhibition of SphK2 in C57BL/6 mouse assessed as Cmax required for two-fold increase in sphingosine-1-phosphate level in blood at 10 mg/kg, ip administered as single dose measured after 4 hrs by LC-MS/MS analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 10. | ALA4357642 | F | In vivo inhibition of SphK2 in C57BL/6 mouse assessed as increase in sphingosine-1-phosphate level in blood at 10 mg/kg, ip administered as single dose and measured at 4 hrs post-treatment by LC-MS/MS analysis relative to control | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 11. | ALA4357643 | F | In vivo inhibition of SphK2 in C57BL/6 mouse assessed as increase in sphingosine-1-phosphate level in blood at 10 mg/kg, ip administered as single dose and measured upto 6 hrs post-treatment by LC-MS/MS analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 12. | ALA4357646 | B | Selectivity index, ratio of EC50 for human SphK1 expressed in Saccharomyces cerevisiae KYA1 to EC50 for human SphK2 expressed in Saccharomyces cerevisiae KYA1 | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4357647 | A | Toxicity against Saccharomyces cerevisiae KYA1 assessed as cell death at >3 uM incubated for 24 to 48 hrs | Saccharomyces cerevisiae | 7 | organism-based format | Scientific Literature | ||
| 14. | ALA4689036 | B | Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 15. | ALA4689037 | B | Inhibition of recombinant human SphK2 assessed as decrease in [33P]SIP production using sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method | Homo sapiens | 6 | single protein format | Scientific Literature |
The page will load shortly, Thanks for your patience!