# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4374950 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked VIM-2 (27 to 266 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
2. | ALA4374951 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked NDM-1 (1 to 270 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
3. | ALA4374952 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked SFH1 (3 to 234 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
4. | ALA4374953 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked GOB-18 (1 to 290 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
5. | ALA4374954 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked KPC-2 (29 to 289 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
6. | ALA4374955 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked TEM-1 (24 to 286 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
7. | ALA4374956 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked AmpC (20 to 377 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
8. | ALA4374957 | Binding | Inhibition of bacterial N-terminal His-tagged TEV protease site linked OXA-48 (1 to 265 amino acids) expressed in Escherichia coli Transetta (DE3) preincubated for 10 mins followed by FC5 fluorescent substrate addition by fluorescence assay | Bacteria | 35 | ALA4373692 | assay format | Scientific Literature | |
9. | ALA4374958 | ADME | Inhibition of serine hydrolase in Escherichia coli Transetta (DE3) cell lysates at 50 uM preincubated for 30 mins followed by TAMRA-FP probe labeling for 20 mins by SDS-PAGE based ABPP analysis | Escherichia coli | 9 | ALA4373692 | subcellular format | Scientific Literature | |
10. | ALA4374959 | ADME | Inhibition of serine hydrolase in HEK293T cell lysates at 50 uM preincubated for 30 mins followed by TAMRA-FP probe labeling for 20 mins by SDS-PAGE based ABPP analysis | Homo sapiens | 9 | ALA4373692 | subcellular format | Scientific Literature | |
11. | ALA4374960 | ADME | Inhibition of human C-terminal His/FLAG-tagged ACE-2 expressed in HEK293 cells at 100 uM preincubated for 10 mins followed by Mca-Ala-Pro-Lys(Dnp)-OH substrate addition by fluorescence assay relative to control | Homo sapiens | 24 | ALA4373692 | cell-based format | Scientific Literature | |
12. | ALA4374961 | Binding | Inhibition of beta lactamase NDM1 in Escherichia coli BAA-2452 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 32 ug/ml) | Escherichia coli | 7 | ALA4373692 | assay format | Scientific Literature | |
13. | ALA4374962 | Binding | Inhibition of beta lactamase NDM1 in Klebsiella pneumoniae 13249 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 64 ug/ml) | Klebsiella pneumoniae | 7 | ALA4373692 | single protein format | Scientific Literature | |
14. | ALA4374963 | Binding | Inhibition of beta lactamase KPC-2 in Escherichia coli BAA-2340 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 4 ug/ml) | Escherichia coli | 7 | ALA4373692 | single protein format | Scientific Literature | |
15. | ALA4374964 | Binding | Inhibition of beta lactamase KPC-2 in Escherichia coli 11119 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 16 ug/ml) | Escherichia coli | 7 | ALA4373692 | single protein format | Scientific Literature | |
16. | ALA4374965 | Binding | Inhibition of beta lactamase KPC-2 in Klebsiella pneumoniae BAA-1705 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 16 ug/ml) | Klebsiella pneumoniae | 7 | ALA4373692 | assay format | Scientific Literature | |
17. | ALA4374966 | Binding | Inhibition of beta lactamase KPC-2 in Klebsiella pneumoniae 5846 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 16 ug/ml) | Klebsiella pneumoniae | 7 | ALA4373692 | assay format | Scientific Literature | |
18. | ALA4374967 | Binding | Inhibition of beta lactamase AmpC in Klebsiella pneumoniae C660 assessed as potentiation of meropenem-induced antibacterial activity by measuring meropenem MIC at 50 uM after 16 to 20 hrs by broth microdilution assay (Rvb = 32 ug/ml) | Klebsiella pneumoniae | 7 | ALA4373692 | assay format | Scientific Literature | |
19. | ALA4374968 | ADME | Cytotoxicity against HEK293T cells up to 100 uM after 72 hrs by MTT assay | Homo sapiens | 5 | ALA4373692 | cell-based format | Scientific Literature | |
20. | ALA4374969 | ADME | Cytotoxicity against human MCF7 cells up to 100 uM after 72 hrs by MTT assay | Homo sapiens | 5 | ALA4373692 | cell-based format | Scientific Literature |