# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4401500 | B | Inhibition of recombinant human G9a using biotinylated-Histone H3 peptide (1 to 21 residues) after 30 mins in presence of SAM by AlphaLISA assay | Homo sapiens | 6 | ALA4400539 | single protein format | Scientific Literature | |
2. | ALA4401501 | B | Binding affinity to human His-tagged G9a expressed in Escherichia coli Rosetta (DE3) after 30 mins by MST binding assay | Homo sapiens | 2 | ALA4400539 | assay format | Scientific Literature | |
3. | ALA4401502 | B | Binding affinity to human His-tagged G9a expressed in Escherichia coli Rosetta (DE3) assessed as association rate constant after 60 secs by SPR assay | Homo sapiens | 2 | ALA4400539 | assay format | Scientific Literature | |
4. | ALA4401503 | B | Binding affinity to human His-tagged G9a expressed in Escherichia coli Rosetta (DE3) assessed as dissociation rate constant after 100 secs by SPR assay | Homo sapiens | 2 | ALA4400539 | assay format | Scientific Literature | |
5. | ALA4401504 | B | Binding affinity to human His-tagged G9a expressed in Escherichia coli Rosetta (DE3) assessed as equilibrium dissociation constant by SPR assay | Homo sapiens | 2 | ALA4400539 | assay format | Scientific Literature | |
6. | ALA4401505 | B | Inhibition of recombinant human G9a at 10 uM using biotinylated-Histone H3 peptide (1 to 21 residues) after 30 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
7. | ALA4401506 | B | Inhibition of recombinant human G9a at 25 uM using biotinylated-Histone H3 peptide (1 to 21 residues) after 30 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
8. | ALA4401507 | B | Inhibition of recombinant human GLP at 10 uM using biotinylated-Histone H3 peptide (1 to 21 residues) after 30 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
9. | ALA4401508 | B | Inhibition of recombinant human GLP at 25 uM using biotinylated-Histone H3 peptide (1 to 21 residues) after 30 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
10. | ALA4401509 | B | Inhibition of recombinant human SETD8 at 10 uM using biotinylated-Histone H4 peptide as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of SAM by AlphaLISA assay relative to control | Homo sapiens | 2 | ALA4400539 | single protein format | Scientific Literature | |
11. | ALA4401510 | B | Inhibition of recombinant human SETD8 at 25 uM using biotinylated-Histone H4 peptide as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of SAM by AlphaLISA assay relative to control | Homo sapiens | 2 | ALA4400539 | single protein format | Scientific Literature | |
12. | ALA4401511 | B | Non-competitive inhibition of recombinant human G9a using biotinylated-Histone H3 peptide (1 to 21 residues) as substrate at varying concentration after 30 mins in presence SAM by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
13. | ALA4401512 | B | Competitive inhibition of recombinant human G9a using biotinylated-Histone H3 peptide (1 to 21 residues) as substrate after 30 mins in presence of varying concentration of SAM by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
14. | ALA4401513 | B | Inhibition of N-terminal GST-tagged recombinant full length human DNMT1 (2 to 1632 residues) using poly(dI-dC)):poly(dI-dC) as substrate preincubated for 20 mins followed by [3H]-SAM and measured after 60 mins by scintillation counting | Homo sapiens | 2 | ALA4400539 | single protein format | Scientific Literature | |
15. | ALA4401514 | B | Inhibition of recombinant human JMJD2A at 10 to 25 uM using biotinylated-Histone H3 peptide (1 to 21 residues) as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of SAM by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
16. | ALA4401515 | B | Inhibition of recombinant human PRMT1 at 10 to 25 uM using biotinylated-Histone H4 peptide (1 to 21 residues) as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of SAM by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
17. | ALA4401516 | B | Inhibition of recombinant human PRMT3 at 10 to 25 uM using biotinylated-Histone H4 peptide (1 to 21 residues) as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of SAM by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
18. | ALA4401517 | B | Inhibition of recombinant human P300 at 10 to 25 uM using biotinylated-Histone H4 peptide (1 to 21 residues) as substrate preincubated for 30 mins followed by substrate addition and measured after 60 mins in presence of SAM by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4400539 | single protein format | Scientific Literature | |
19. | ALA4401518 | P | Chemical stability of the compound in PBS buffer after 72 hrs by HPLC analysis | 1 | ALA4400539 | small-molecule physicochemical format | Scientific Literature | ||
20. | ALA4401519 | A | Metabolic stability in human liver microsomes assessed as parent compound turn over percentage at 1 mM preincubated for 5 mins followed by NADPH addition and measured for 60 mins by UHPLC analysis | Homo sapiens | 2 | ALA4400539 | microsome format | Scientific Literature |