#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4428139	B	Activation of full-length GST-tagged recombinant human SIRT1 assessed as enzyme activity obtained without subtraction of intrinsic fluorescence using H3K9 as substrate at 50 uM after 30 mins by SIRTainty assay	Homo sapiens	56	ALA4428023	single protein format	Scientific Literature
2.	ALA4428140	B	Activation of full-length GST-tagged recombinant human SIRT1 assessed as enzyme activity obtained with subtraction of intrinsic fluorescence using H3K9 as substrate at 50 uM after 30 mins by SIRTainty assay	Homo sapiens	51	ALA4428023	single protein format	Scientific Literature
3.	ALA5112701	B	Activation of SIRT3 in human MDA-MB-231 cells assessed as reduction in GDH level at 50 uM incubated for 4 to 24 hrs	Homo sapiens	1	ALA5108071	cell-based format	Scientific Literature
4.	ALA5112702	B	Activation of SIRT3 in human MDA-MB-231 cells assessed as blockade of ammonia accumulation at 50 uM incubated for 4 to 24 hrs	Homo sapiens	1	ALA5108071	cell-based format	Scientific Literature
5.	ALA5112704	B	Activation of SIRT3 in human S2-013 cells assessed as decrease in GOT1 acetylation at 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	3	ALA5108071	cell-based format	Scientific Literature
6.	ALA5112707	B	Activation of N-terminal SUMO-tagged human SIRT2 (43 to 356 residues) expressed in Escherichia coli assessed as fold activation at 100 uM using acetylated alpha tubulin-K25 as substrate in presence of NAD+	Homo sapiens	43	ALA5108071	assay format	Scientific Literature
7.	ALA5112710	B	Activation of N-terminal his-tagged human SIRT5 (34 to 302 residues) expressed in Escherichia coli assessed as fold activation at 100 uM using CPS1-succ537 as substrate in presence of NAD+	Homo sapiens	13	ALA5108071	assay format	Scientific Literature
8.	ALA5112711	B	Activation of N-terminal his-tagged human SIRT5 (34 to 302 residues) expressed in Escherichia coli assessed as fold activation at 10 uM using CPS1-succ537 as substrate in presence of NAD+	Homo sapiens	7	ALA5108071	assay format	Scientific Literature
9.	ALA5112743	B	Activation of SIRT3 in human CAPAN-1 cells assessed as decrease in GOT1 acetylation at 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	3	ALA5108071	cell-based format	Scientific Literature
10.	ALA5141125	B	Activation of SIRT5 (unknown origin) assessed as fold increase in deacetylase activity at 10 uM relative to control	Homo sapiens	1	ALA5137064	single protein format	Scientific Literature
11.	ALA5141126	B	Activation of SIRT5 (unknown origin) assessed as fold increase in deacetylase activity at 50 uM relative to control	Homo sapiens	1	ALA5137064	single protein format	Scientific Literature
12.	ALA5141127	B	Activation of SIRT5 (unknown origin) assessed as fold increase in deacetylase activity at 200 uM relative to control	Homo sapiens	1	ALA5137064	single protein format	Scientific Literature
13.	ALA5141128	F	Cytotoxicity against human PDAC cells assessed as cell viability	Homo sapiens	1	ALA5137064	cell-based format	Scientific Literature