# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4376273 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring change in melting temperature at 0.1 mM by SYPRO orange based thermal shift assay | Pseudomonas aeruginosa | 11 | ALA4373727 | cell-based format | Scientific Literature | |
2. | ALA4376310 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as reduction in S-adenosyl-L-homocysteine production up to 50 uM preincubated for 15 mins followed by 1.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 8 | ALA4373727 | cell-based format | Scientific Literature |