# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4397816 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclast differentiation by measuring decrease in multinucleated TRAP+ cells supplemented with fresh medium containing compounds every 2 days for 5 to 6 days by TRAP staining-based microscopic analysis | Mus musculus | 5 | ALA4396880 | cell-based format | Scientific Literature | |
2. | ALA4397817 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclastogenesis by measuring reduction in TRAP activity at 5 uM measured after 3 days by colorimetric assay relative to control | Mus musculus | 37 | ALA4396880 | protein format | Scientific Literature | |
3. | ALA4397818 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclastogenesis by measuring reduction in cathepsin K mRNA level at 2.5 uM measured after 3 days by Trizol-reagent-based qRT-PCR analysis | Mus musculus | 1 | ALA4396880 | protein format | Scientific Literature | |
4. | ALA4397821 | B | Binding affinity to human RANKL by surface plasmon resonance analysis | Homo sapiens | 7 | ALA4396880 | single protein format | Scientific Literature | |
5. | ALA4397823 | A | Cytotoxicity against M-CSF-stimulated C57/BL6 mouse bone marrow macrophages assessed as cell viability at 10 uM measured after 2 days by MTT assay (Rvb = 100%) | Mus musculus | 4 | ALA4396880 | cell-based format | Scientific Literature | |
6. | ALA4397824 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclast differentiation by measuring decrease in multinucleated TRAP+ cells at 5 uM supplemented with fresh medium containing compounds every 2 days for 5 to 6 days by TRAP staining-based microscopic analysis | Mus musculus | 4 | ALA4396880 | cell-based format | Scientific Literature | |
7. | ALA4397825 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclast differentiation by measuring decrease in nuclei size of F-actin ring formation at 5 uM supplemented with fresh medium containing compounds every 2 days for 5 to 6 days by rhodamine phalloidin/DAPI staining-based microscopic analysis | Mus musculus | 4 | ALA4396880 | protein format | Scientific Literature | |
8. | ALA4397826 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclast differentiation by measuring decrease in nuclei number of F-actin ring formation at 5 uM supplemented with fresh medium containing compounds every 2 days for 5 to 6 days by rhodamine phalloidin/DAPI staining-based microscopic analysis | Mus musculus | 4 | ALA4396880 | protein format | Scientific Literature | |
9. | ALA4397827 | B | Binding affinity to human TNFalpha by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4396880 | single protein format | Scientific Literature | |
10. | ALA4397828 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced bone resorption at 1 to 5 uM measured after 2 days by toluidine blue staining-based confocal microscopic analysis | Mus musculus | 1 | ALA4396880 | protein format | Scientific Literature | |
11. | ALA4397830 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclastogenesis by measuring reduction in TRAP mRNA level at 2.5 uM measured after 3 days by Trizol-reagent-based qRT-PCR analysis | Mus musculus | 1 | ALA4396880 | protein format | Scientific Literature | |
12. | ALA4397831 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclastogenesis by measuring reduction in NFATc1 mRNA level at 2.5 uM measured after 3 days by Trizol-reagent-based qRT-PCR analysis | Mus musculus | 1 | ALA4396880 | protein format | Scientific Literature | |
13. | ALA4397832 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclastogenesis by measuring reduction in NFATc1 expression measured after 3 days by Western blot analysis | Mus musculus | 1 | ALA4396880 | assay format | Scientific Literature | |
14. | ALA4397833 | B | Inhibition of RANKL-RANK protein-protein interaction in C57/BL6 mouse bone marrow macrophage assessed as suppression of M-CSF/mRANKL-induced osteoclastogenesis by measuring reduction in c-fos expression measured after 3 days by Western blot analysis | Mus musculus | 1 | ALA4396880 | assay format | Scientific Literature | |
15. | ALA5126259 | F | Anti-osteoporosis activity C57/BL6 mouse bone marrow macrophage cells assessed as inhibition of RANKL-induced osteoclastogenesis at 1 uM measured after 5 to 6 days in presence of M-CSF and RNKL by TRAP staining microscopic analysis | Mus musculus | 40 | ALA5120967 | cell-based format | Scientific Literature | |
16. | ALA5126261 | T | Cytotoxicity against C57/BL6 mouse bone marrow macrophage cells assessed as reduction in cell viability at 10 uM incubated for 3 days by CCK8 assay | Mus musculus | 43 | ALA5120967 | cell-based format | Scientific Literature | |
17. | ALA5126275 | A | AUC(0 to t) in OVX-induced C57/BL6 mouse model at 3 mg/kg, iv | Mus musculus | 2 | ALA5120967 | organism-based format | Scientific Literature | |
18. | ALA5126276 | A | Half life in OVX-induced C57/BL6 mouse model at 3 mg/kg, iv | Mus musculus | 2 | ALA5120967 | organism-based format | Scientific Literature | |
19. | ALA5126277 | A | Clearance in OVX-induced C57/BL6 mouse model at 3 mg/kg, iv | Mus musculus | 2 | ALA5120967 | organism-based format | Scientific Literature | |
20. | ALA5126278 | A | Oral bioavailability in OVX-induced C57/BL6 mouse model | Mus musculus | 2 | ALA5120967 | organism-based format | Scientific Literature |