# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4402109 | B | Inhibition of His-tagged p97 (unknown origin) in presence of ATP and PNP by UV-transparent microplate assay | Homo sapiens | 19 | ALA4400565 | single protein format | Scientific Literature | |
2. | ALA4402110 | P | GSH reactivity in PBS buffer assessed as half life at 1 uM after presence of GSH by LC-MS/MS analysis | 13 | ALA4400565 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA4402111 | F | Antiproliferative activity against human MIAPaCa2 cells | Homo sapiens | 19 | ALA4400565 | cell-based format | Scientific Literature | |
4. | ALA4402112 | B | Irreversible inhibition of His-tagged p97 (unknown origin) using PNP as substrate assessed as activity recovered at 780 nM after 2 hrs by dialysis relative to control | Homo sapiens | 1 | ALA4400565 | single protein format | Scientific Literature | |
5. | ALA4402113 | B | Irreversible inhibition of His-tagged p97 C552A mutant (unknown origin) using PNP as substrate assessed as activity recovered at 780 nM after 2 hrs by dialysis relative to control | Homo sapiens | 1 | ALA4400565 | single protein format | Scientific Literature | |
6. | ALA4402114 | B | Irreversible inhibition of His-tagged p97 delta N mutant (unknown origin) using PNP as substrate assessed as activity recovered at 780 nM after 2 hrs by dialysis relative to control | Homo sapiens | 1 | ALA4400565 | assay format | Scientific Literature | |
7. | ALA4402118 | B | Inhibition of p97 ATPase activity in human HEK293T cells based Ubv-GFP cells assessed as increase Ub-v-GFP accumulation at 2 uM after 4 hrs by Western blot analysis | Homo sapiens | 14 | ALA4400565 | cell-based format | Scientific Literature | |
8. | ALA4402123 | B | Irreversible inhibition of p97 ATPase activity in human HEK293T cells based Ubv-GFP cells assessed as increase Ub-v-GFP accumulation at 2 uM incubated for 1 hr followed by culturing in drug-free medium for additional 3 hrs by Western blot analysis | Homo sapiens | 5 | ALA4400565 | cell-based format | Scientific Literature | |
9. | ALA4402124 | B | Irreversible inhibition of p97 ATPase activity in human HEK293T cells based Ubv-GFP cells assessed as increase Ub-v-GFP accumulation at 2 uM incubated for 4 hrs by Western blot analysis | Homo sapiens | 5 | ALA4400565 | cell-based format | Scientific Literature | |
10. | ALA4402125 | B | Inhibition of p97 in human A373 cells co-expressing US11 and HA-tagged MCH class 1 heavy chain H2A assessed as MHC class 1 heavy chain accumulation at 2 uM after 1 hr by Western blot analysis | Homo sapiens | 2 | ALA4400565 | cell-based format | Scientific Literature | |
11. | ALA4402127 | B | Noncompetitive inhibition of p97 (unknown origin) using increasing concentration of ATP preincubated for 10 mins followed by ATP addition and measured after 2 hrs by UV-based two-step endpoint assay | Homo sapiens | 1 | ALA4400565 | single protein format | Scientific Literature | |
12. | ALA4402128 | P | GSH reactivity in PBS buffer assessed as GSH adduct formation at 1 uM after presence of GSH by LC-MS/MS analysis | 13 | ALA4400565 | small-molecule physicochemical format | Scientific Literature | ||
13. | ALA4402131 | B | Inhibition of proteasome in human HEK293T cells stably expressing YFP delSS TCRalpha assessed as reduction in deltaSS TCRalpha-YFP degradation at 2 uM MG132 incubated overnight by Western blot analysis | Homo sapiens | 2 | ALA4400565 | subcellular format | Scientific Literature | |
14. | ALA4402134 | B | Irreversible inhibition of p97 in human A373 cells co-expressing US11 and HA-tagged MCH class 1 heavy chain H2A assessed as half life for MHC class 1 heavy chain degradation at 2 uM incubated for 1 hr followed by further incubation in drug-free medium in presence of cycloheximide by Western blot analysis | Homo sapiens | 2 | ALA4400565 | cell-based format | Scientific Literature | |
15. | ALA4402135 | B | Irreversible inhibition of p97 in human A373 cells co-expressing US11 and HA-tagged MCH class 1 heavy chain H2A assessed as MHC class 1 heavy chain degradation at 0.5 uM incubated for 1 hr followed by further incubation in drug-free medium in presence of cycloheximide by Western blot analysis | Homo sapiens | 1 | ALA4400565 | cell-based format | Scientific Literature | |
16. | ALA4402137 | B | Binding affinity to p97 (unknown origin) assessed as covalent modification at Cys535 residues at 10 uM by SDS-PAGE and LC-MS/MS analysis | Homo sapiens | 2 | ALA4400565 | single protein format | Scientific Literature | |
17. | ALA4402138 | B | Binding affinity to p97 (unknown origin) assessed as covalent modification at Cys522 residues at 10 uM by SDS-PAGE and LC-MS/MS analysis | Homo sapiens | 2 | ALA4400565 | single protein format | Scientific Literature | |
18. | ALA4402139 | B | Binding affinity to p97 (unknown origin) assessed as covalent modification at Cys105 residues at 10 uM by SDS-PAGE and LC-MS/MS analysis | Homo sapiens | 2 | ALA4400565 | single protein format | Scientific Literature |