| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4378764 | B | Inhibition of FITC-labeled YAP peptide (61 to 100 residues) binding to TEAD4 (unknown origin) preincubated for 1 hr followed by FITC-labeled YAP peptide addition and measured after after 30 mins by fluorescence polarization assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 2. | ALA4378770 | B | Inhibition of N-terminal His6 tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as reduction in autopalmitoylation at 800 nM preincubated for 2 hrs followed by palmitoyl alkyne-coenzyme A addition and measured after 30 mins by immunoblot analysis relative to control | Homo sapiens | 12 | ALA4376829 | cell-based format | Scientific Literature | |
| 3. | ALA4378771 | B | Inhibition of N-terminal His6 tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as reduction in autopalmitoylation at 200 nM preincubated for 2 hrs followed by palmitoyl alkyne-coenzyme A addition and measured after 30 mins immunoblot analysis relative to control | Homo sapiens | 12 | ALA4376829 | cell-based format | Scientific Literature | |
| 4. | ALA4378772 | B | Inhibition of N-terminal His6 tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as reduction in autopalmitoylation preincubated for 2 hrs followed by palmitoyl alkyne-coenzyme A addition and measured after 30 mins by immunoblot analysis | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 5. | ALA4378774 | B | Binding affinity to N-terminal His6 tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as covalent modification by measuring change in protein mass by mass spectral analysis | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 6. | ALA4378775 | B | Binding affinity to N-terminal His6 tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as covalent modification at Cys367 residue by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 7. | ALA4378776 | B | Binding affinity to N-terminal His6-tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as change in melting temperature at 1:1 ratio by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 8. | ALA4378777 | B | Binding affinity to N-terminal His6-tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as change in melting temperature at 1:2 ratio by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 9. | ALA4378778 | B | Binding affinity to N-terminal His6-tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells assessed as change in melting temperature at 1:4 ratio by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 10. | ALA4378780 | B | Binding affinity to TEAD4-YBD C367A mutant (unknown origin) assessed as change in melting temperature by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 11. | ALA4378781 | B | Binding affinity to TEAD4-YBD C335A mutant (unknown origin) assessed as change in melting temperature by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | assay format | Scientific Literature | |
| 12. | ALA4378782 | B | Binding affinity to TEAD4-YBD C330A mutant (unknown origin) assessed as change in melting temperature by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 13. | ALA4378783 | B | Binding affinity to TEAD4-YBD C410A mutant (unknown origin) assessed as change in melting temperature by SYPRO-dye based thermal shift assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 14. | ALA4378784 | U | CoA reactivity in PBS assessed as CoA adduct formation at 1 mM after 8 hrs by UPLC-MS analysis | 1 | ALA4376829 | assay format | Scientific Literature | ||
| 15. | ALA4378785 | P | GSH reactivity in PBS assessed as GSH adduct formation at 1 mM after 8 hrs by UPLC-MS analysis | 1 | ALA4376829 | small-molecule physicochemical format | Scientific Literature | ||
| 16. | ALA4378786 | B | Inhibition of N-terminal His6-tagged human TEAD4-YBD (217 to 434 residues) expressed in Escherichia coli C43 (DE3) cells at 50 to 1000 nM preincubated for 2 hrs followed by palmitoyl alkyne-coenzyme A addition and measured after 30 mins in presence of BSA by immunoblot analysis | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature | |
| 17. | ALA4378787 | B | Binding affinity to FABP3 (unknown origin) at 1:1 ratio by fluorescence based thermal shift assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 18. | ALA4378788 | B | Binding affinity to FABP4 (unknown origin) at 1:1 ratio by fluorescence based thermal shift assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 19. | ALA4378789 | B | Binding affinity to FABP5 (unknown origin) at 1:1 ratio by fluorescence based thermal shift assay | Homo sapiens | 1 | ALA4376829 | single protein format | Scientific Literature | |
| 20. | ALA4378790 | B | Inhibition of TEAD (unknown origin) expresssed in HEK293T cells assessed as reduction in luciferase activity at 10 to 20 uM by dual-luciferase reporter gene assay | Homo sapiens | 1 | ALA4376829 | cell-based format | Scientific Literature |
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