# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4328666 | B | Inhibition of human GST-tagged catalytic ROCK1 expressed in baculovirus system using STK S2 peptide substrate and and 33P-ATP after 60 mins by HTRF assay | Homo sapiens | 44 | ALA4325872 | assay format | Scientific Literature | |
2. | ALA4328667 | B | Inhibition of GST-fused human recombinant ROCK2 (11 to 552 residues) expressed in Spodoptera frugiperda insect cells using STK S2 peptide substrate and 33P-ATP after 60 mins by HTRF assay | Homo sapiens | 45 | ALA4325872 | cell-based format | Scientific Literature | |
3. | ALA4328668 | B | Inhibition of PKA (unknown origin) | Homo sapiens | 43 | ALA4325872 | assay format | Scientific Literature | |
4. | ALA4328670 | P | Solubility of compound by high throughput assay | 13 | ALA4325872 | small-molecule physicochemical format | Scientific Literature | ||
5. | ALA4328671 | A | Protein binding in plasma (unknown origin) assessed as unbound fraction | Not specified | 14 | ALA4325872 | cell-free format | Scientific Literature | |
6. | ALA4328672 | A | Permeability of compound by PAMPA | 16 | ALA4325872 | assay format | Scientific Literature | ||
7. | ALA4328673 | A | Inhibition of C-terminal 4His-tagged CYP2C9 (unknown origin) expressed in Escherichia coli by spectrophotometric analysis | Homo sapiens | 11 | ALA4325872 | assay format | Scientific Literature | |
8. | ALA4328674 | A | Inhibition of C-terminal 4His-tagged CYP2D6 (unknown origin) expressed in Escherichia coli by spectrophotometric analysis | Homo sapiens | 10 | ALA4325872 | assay format | Scientific Literature | |
9. | ALA4328675 | A | Inhibition of C-terminal 4His-tagged CYP3A4 (unknown origin) expressed in Escherichia coli by spectrophotometric analysis | Homo sapiens | 11 | ALA4325872 | assay format | Scientific Literature | |
10. | ALA4328676 | B | Inhibition of ROCK1/2 in human PANC1 cells assessed as reduction in MYPT1 phosphorylation after 1 hr by ELISA | Homo sapiens | 14 | ALA4325872 | cell-based format | Scientific Literature | |
11. | ALA4328708 | P | Thiol reactivity assessed as DTT-dependent (13)C chemical shift changes of human La antigen by ALARM NMR assay | 2 | ALA4325872 | small-molecule physicochemical format | Scientific Literature | ||
12. | ALA5136851 | B | Inhibition of recombinant N-terminal GST-tagged human ROCK2 (5 to 554 residues) expressed in baculovirus infected Sf9 cells using S6K as substrate incubated for 30 mins in presence of ATP by ADP Glo kinase assay | Homo sapiens | 33 | ALA5131572 | cell-based format | Scientific Literature | |
13. | ALA5136852 | B | Inhibition of ROCK in rat A7R5 cells assessed as cofilin serine 3 phosphorylation incubated for 1 hr by ICW assay | Rattus norvegicus | 33 | ALA5131572 | cell-based format | Scientific Literature | |
14. | ALA5136853 | P | Kinetic solubility of the compound in pH 7.4 PBS buffer incubated for 2 hrs using orbital shaker by LC-UV mass spectrometry | 33 | ALA5131572 | small-molecule physicochemical format | Scientific Literature | ||
15. | ALA5136854 | P | Distribution coefficient, logD of compound by chromatographic method | 13 | ALA5131572 | small-molecule physicochemical format | Scientific Literature | ||
16. | ALA5136855 | P | Lipophilicity, logP of compound | 13 | ALA5131572 | small-molecule physicochemical format | Scientific Literature | ||
17. | ALA5136856 | A | Efflux ratio of apparent permeability across basolateral to apical side to apical to basolateral side in MDCK-MDR1 cells at 10 uM measured after 1 hr by LC-MS/MS analysis | Canis lupus familiaris | 29 | ALA5131572 | cell-based format | Scientific Literature | |
18. | ALA5136857 | A | Apparent permeability in MDCK-MDR1 cells at 10 uM after 1 hr by LC-MS/MS analysis | Canis lupus familiaris | 29 | ALA5131572 | cell-based format | Scientific Literature | |
19. | ALA5136858 | A | Metabolic stability in mouse liver microsomes assessed as intrinsic clearance at 1 uM measured upto 40 mins in presence of NADPH by LC-MS/MS analysis | Mus musculus | 29 | ALA5131572 | microsome format | Scientific Literature | |
20. | ALA5136859 | B | Inhibition of PKA (unknown origin) | Homo sapiens | 4 | ALA5131572 | protein format | Scientific Literature |