Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric affinity cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay
Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring efficacy cooperativity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay
Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric binding affinity in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay
Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production by measuring allosteric index in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay
Positive allosteric modulation of human GPR68 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay
Positive allosteric modulation of human GPR4 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay
Positive allosteric modulation of human GPR65 expressed in HEK293T cells co-expressing Gs assessed as stimulation of proton-mediated cAMP production in HBSS buffer containing pH 6.8 to 8.2 HEPES/pH 8.3 to 8.6 TAPS measured after 20 to 30 mins in presence of PDE inhibitor Ro 20-1724 by luciferase-glo reporter gene assay