# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4397534 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 12.5 to 200 nM at 10 to 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 3 | ALA4396874 | cell-based format | Scientific Literature | |
2. | ALA4397535 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 10 to 25 degreeC by surface plasmon resonance analysis relative to control | Homo sapiens | 5 | ALA4396874 | cell-based format | Scientific Literature | |
3. | ALA4397536 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 10 to 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 4 | ALA4396874 | cell-based format | Scientific Literature | |
4. | ALA4397537 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 7 | ALA4396874 | cell-based format | Scientific Literature | |
5. | ALA4397538 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 6 | ALA4396874 | cell-based format | Scientific Literature | |
6. | ALA4397539 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 25 degreeC by surface plasmon resonance analysis | Homo sapiens | 6 | ALA4396874 | cell-based format | Scientific Literature | |
7. | ALA4397540 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant at 25 degreeC in presence 225 to 1000 mM of NaCl by surface plasmon resonance analysis | Homo sapiens | 3 | ALA4396874 | cell-based format | Scientific Literature | |
8. | ALA4397541 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant at 25 degreeC in presence 225 to 1000 mM of NaCl by surface plasmon resonance analysis | Homo sapiens | 3 | ALA4396874 | cell-based format | Scientific Literature | |
9. | ALA4397542 | B | Binding affinity to N-terminal 10His-tagged m7GDP-bound recombinant human eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant at 25 degreeC in presence 225 to 1000 mM of NaCl by surface plasmon resonance analysis | Homo sapiens | 2 | ALA4396874 | cell-based format | Scientific Literature | |
10. | ALA4397543 | B | Inhibition of Halo-tagged biotinylated human eIF4E expressed in Escherichia coli/Halo-tagged methyltetrazine-labelled human 4E-BP1 expressed in Escherichia coli protein-protein interaction incubated for 1 hr by catalytic enzyme-linked click chemistry assay | Homo sapiens | 7 | ALA4396874 | assay format | Scientific Literature | |
11. | ALA4773310 | B | Inhibition of recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells by catalytic enzyme-linked click chemistry assay | Homo sapiens | 3 | ALA4765410 | cell-based format | Scientific Literature | |
12. | ALA4773311 | B | Binding affinity to recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant by surface plasmon resonance analysis | Homo sapiens | 3 | ALA4765410 | cell-based format | Scientific Literature | |
13. | ALA4773312 | B | Binding affinity to recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by surface plasmon resonance analysis | Homo sapiens | 3 | ALA4765410 | cell-based format | Scientific Literature | |
14. | ALA4773313 | B | Binding affinity to recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant by surface plasmon resonance analysis | Homo sapiens | 3 | ALA4765410 | cell-based format | Scientific Literature |