| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4397429 | B | Inhibition of N-terminal GST-tagged human PDE5A1 expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 18 | ALA4396872 | cell-based format | Scientific Literature | |
| 2. | ALA4397430 | F | Antihypertensive activity in spontaneously hypertensive rat assessed as reduction in mean arterial pressure at 20 mg/kg administered via single gavage measured up to 24 hrs | Rattus norvegicus | 2 | ALA4396872 | organism-based format | Scientific Literature | |
| 3. | ALA4397431 | B | Selectivity ratio of IC50 for N-terminal GST-tagged human PDE6C to IC50 for N-terminal GST-tagged human PDE5A1 | Homo sapiens | 18 | ALA4396872 | assay format | Scientific Literature | |
| 4. | ALA4397432 | A | Metabolic stability in human liver microsomes assessed as parent compound remaining at 1 uM in presence of NADPH after 30 mins by LC-MS/MS analysis | Homo sapiens | 4 | ALA4396872 | microsome format | Scientific Literature | |
| 5. | ALA4397433 | B | Binding affinity to recombinant human PDE5A1 catalytic domain (535 to 860 residues) expressed in Escherichia coli BL21 DE3 Rosetta2 pLysS cells assessed as dissociation constant at 200 to 250 uM by by isothermal titration calorimetry | Homo sapiens | 2 | ALA4396872 | cell-based format | Scientific Literature | |
| 6. | ALA4397434 | B | Binding affinity to recombinant human PDE5A1 catalytic domain (535 to 860 residues) expressed in Escherichia coli BL21 DE3 Rosetta2 pLysS cells assessed as change in gibbs free energy at 200 to 250 uM by by isothermal titration calorimetry | Homo sapiens | 2 | ALA4396872 | cell-based format | Scientific Literature | |
| 7. | ALA4397435 | B | Binding affinity to recombinant human PDE5A1 catalytic domain (535 to 860 residues) expressed in Escherichia coli BL21 DE3 Rosetta2 pLysS cells assessed as change in enthalpy at 200 to 250 uM by by isothermal titration calorimetry | Homo sapiens | 2 | ALA4396872 | cell-based format | Scientific Literature | |
| 8. | ALA4397436 | B | Binding affinity to recombinant human PDE5A1 catalytic domain (535 to 860 residues) expressed in Escherichia coli BL21 DE3 Rosetta2 pLysS cells assessed as change in entropy at 200 to 250 uM by by isothermal titration calorimetry | Homo sapiens | 2 | ALA4396872 | cell-based format | Scientific Literature | |
| 9. | ALA4397437 | A | Inhibition of N-terminal GST-tagged human PDE1 expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 10. | ALA4397438 | A | Inhibition of human PDE2A1 expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 11. | ALA4397439 | A | Inhibition of N-terminal GST-tagged human PDE3 expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 12. | ALA4397440 | A | Inhibition of N-terminal GST-tagged human PDE4 expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 13. | ALA4397441 | A | Inhibition of N-terminal GST-tagged human PDE6C expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 14. | ALA4397442 | A | Inhibition of N-terminal GST-tagged human PDE7 expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 15. | ALA4397443 | A | Inhibition of N-terminal GST-tagged full length human PDE8A1 expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 16. | ALA4397444 | A | Inhibition of N-terminal GST-tagged full length human PDE9A2 expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 17. | ALA4397445 | A | Inhibition of N-terminal GST-tagged human PDE10A expressed in baculovirus infected sf9 cells using [3H]cAMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 18. | ALA4397446 | A | Inhibition of N-terminal GST-tagged human PDE11A4 expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate measured after 30 mins by scintillation proximity assay | Homo sapiens | 3 | ALA4396872 | cell-based format | Scientific Literature | |
| 19. | ALA4397447 | B | Selectivity ratio of IC50 for N-terminal GST-tagged human PDE1 to IC50 for N-terminal GST-tagged human PDE5A1 | Homo sapiens | 3 | ALA4396872 | assay format | Scientific Literature | |
| 20. | ALA4397448 | B | Selectivity ratio of IC50 for N-terminal GST-tagged human PDE2A1 to IC50 for N-terminal GST-tagged human PDE5A1 | Homo sapiens | 3 | ALA4396872 | assay format | Scientific Literature |
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