Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as retention time in the presence of NADPH at 100 uM by HPLC analysis (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as half life in the presence of NADPH at 100 uM by HPLC analysis (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as product formation after 10 mins in the presence of NADPH at 100 uM by HPLC analysis relative to control (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)
Cytotoxicity against human PC3 cells assessed as reduction in cell viability measured after 48 hrs in presence of Staphylococcus saprophyticus nitroreductase and NADH by SRB assay
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as retention time in the presence of NADPH at 100 uM by HPLC analysis (0 to 5 mins at 20% ACN, 25 to 35 min at 80% ACN, 40 to 45 min at 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as half life in the presence of NADPH at 100 uM by HPLC analysis (0 to 5 mins at 20% ACN, 25 to 35 min at 80% ACN, 40 to 45 min at 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as product formation after 10 mins in the presence of NADPH at 100 uM by HPLC analysis relative to control (0 to 5 mins at 20% ACN, 25 to 35 min at 80% ACN, 40 to 45 min at 20% ACN)