Prodrug conversion assessed as Staphylococcus saprophyticus nitroreductase mediated P2 metabolite formation in presence of FDH cofactor regeneration system at 100 uM by HPLC analysis
Substrate activity at Staphylococcus saprophyticus nitroreductase assessed as Kcat using NADPH as cofactor in presence of Tris-Cl buffer at pH 7.5 by spectrophotometric method
Substrate activity at Staphylococcus saprophyticus nitroreductase assessed as Km using NADPH as cofactor in presence of Tris-Cl buffer at pH 7.5 by spectrophotometric method
Substrate activity at Staphylococcus saprophyticus nitroreductase assessed as ratio of Kcat/Km using NADPH as cofactor in presence of Tris-Cl buffer at pH 7.5 by spectrophotometric method
Substrate activity at Staphylococcus saprophyticus nitroreductase assessed as ratio of Kcat/Km up to 80 mM using NADH as cofactor relative to CB1954/NfsB
Prodrug conversion assessed as Staphylococcus saprophyticus nitroreductase mediated P1 metabolite formation in presence of FDH cofactor regeneration system at 100 uM by HPLC analysis
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as retention time in the presence of NADPH at 150 uM by HPLC analysis (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as half life in the presence of NADPH at 150 uM by HPLC analysis (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as product formation after 10 mins in the presence of NADPH at 150 uM by HPLC analysis relative to control (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as retention time in the presence of NADPH at 150 uM by HPLC analysis (0 to 5 mins at 20% ACN, 25 to 35 min at 80% ACN, 40 to 45 min at 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as half life in the presence of NADPH at 150 uM by HPLC analysis (0 to 5 mins at 20% ACN, 25 to 35 min at 80% ACN, 40 to 45 min at 20% ACN)
Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as product formation after 10 mins in the presence of NADPH at 150 uM by HPLC analysis relative to control (0 to 5 mins at 20% ACN, 25 to 35 min at 80% ACN, 40 to 45 min at 20% ACN)
Prodrug conversion assessed as Staphylococcus saprophyticus nitroreductase mediated P3 metabolite formation in presence of FDH cofactor regeneration system at 100 uM by HPLC analysis
Prodrug conversion assessed as Staphylococcus saprophyticus nitroreductase mediated P4 metabolite formation in presence of FDH cofactor regeneration system at 100 uM by HPLC analysis
Prodrug conversion assessed as Staphylococcus saprophyticus nitroreductase mediated P5 metabolite formation in presence of FDH cofactor regeneration system at 100 uM by HPLC analysis