# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4344571 | B | Agonist activity at human PPARalpha expressed in nonhuman mammalian cells assessed as increase in receptor transcriptional activity incubated for 22 to 24 hrs by luciferase reporter gene assay | Homo sapiens | 30 | ALA4342468 | cell-based format | Scientific Literature | |
2. | ALA4344574 | B | Selectivity index, ratio of EC50 for human PPARgamma expressed in nonhuman mammalian cells to EC50 for human PPARalpha expressed in nonhuman mammalian cells measured after 22 to 24 hrs by luciferase reporter gene assay | Homo sapiens | 6 | ALA4342468 | cell-based format | Scientific Literature | |
3. | ALA4344575 | B | Selectivity index, ratio of EC50 for human PPARdelta expressed in nonhuman mammalian cells to EC50 for human PPARalpha expressed in nonhuman mammalian cells measured after 22 to 24 hrs by luciferase reporter gene assay | Homo sapiens | 6 | ALA4342468 | cell-based format | Scientific Literature | |
4. | ALA4344576 | B | Inhibition of human ERG stably expressed in CHOK1 cells assessed as tail current amplitude at 10 uM incubated for 5 mins measured after deploarization to +20 mV for 2s followed by 1s pulse to -40 mV with holding potential of -80 mV by Qpatch clamp assay relative to control | Homo sapiens | 2 | ALA4342468 | cell-based format | Scientific Literature | |
5. | ALA4344577 | B | Binding affinity to PPARalpha in human MIO-M1 cells assessed as increase in thermal stability preincubated for 3 hrs followed by exposure to 45.5 degree C for 10 mins by CETSA | Homo sapiens | 2 | ALA4342468 | cell-based format | Scientific Literature | |
6. | ALA4344578 | B | Binding affinity to His-tagged PPARalpha LBD (unknown origin) expressed in Escherichia coli Rosetta (DE3) at 25 degree C by isothermal titration calorimetry | Homo sapiens | 4 | ALA4342468 | assay format | Scientific Literature | |
7. | ALA4344579 | B | Binding affinity to His-tagged PPARalpha LBD (unknown origin) expressed in Escherichia coli Rosetta (DE3) assessed as change in Gibbs free energy at 25 degree C by isothermal titration calorimetry | Homo sapiens | 4 | ALA4342468 | assay format | Scientific Literature | |
8. | ALA4344580 | B | Binding affinity to His-tagged PPARalpha LBD (unknown origin) expressed in Escherichia coli Rosetta (DE3) assessed as change in enthalpy at 25 degree C by isothermal titration calorimetry | Homo sapiens | 4 | ALA4342468 | assay format | Scientific Literature | |
9. | ALA4344581 | B | Binding affinity to His tagged PPARalpha LBD (unknown origin) expressed in Escherichia coli Rosetta (DE3) assessed as change in entropy at 25 degree C by isothermal titration calorimetry | Homo sapiens | 4 | ALA4342468 | assay format | Scientific Literature | |
10. | ALA4344582 | A | Stability in Sprague Dawley rat pooled liver microsomes preincubated for 5 mins prior to addition of NADPH-regenerating system and measured up to 60 mins by HPLC-MS/MS analysis | Rattus norvegicus | 1 | ALA4342468 | microsome format | Scientific Literature | |
11. | ALA4344583 | A | Stability in human pooled liver microsomes preincubated for 5 mins prior to addition of NADPH-regenerating system and measured up to 60 mins by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4342468 | microsome format | Scientific Literature | |
12. | ALA4344584 | A | Irreversible inhibition of CYP1A in human liver microsomes at 10 uM preincubated for 30 mins in presence of NADPH-generating system followed by substrate addition and measured after 10 mins by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4342468 | microsome format | Scientific Literature | |
13. | ALA4344585 | A | Irreversible inhibition of CYP2C9 in human liver microsomes at 10 uM preincubated for 30 mins in presence of NADPH-generating system followed by substrate addition and measured after 10 mins by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4342468 | microsome format | Scientific Literature | |
14. | ALA4344586 | A | Irreversible inhibition of CYP2C19 in human liver microsomes at 10 uM preincubated for 30 mins in presence of NADPH-generating system followed by substrate addition and measured after 10 mins by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4342468 | microsome format | Scientific Literature | |
15. | ALA4344587 | A | Irreversible inhibition of CYP2D6 in human liver microsomes at 10 uM preincubated for 30 mins in presence of NADPH-generating system followed by substrate addition and measured after 10 mins by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4342468 | microsome format | Scientific Literature | |
16. | ALA4344588 | A | Irreversible inhibition of CYP3A in human liver microsomes at 10 uM preincubated for 30 mins in presence of NADPH-generating system followed by substrate addition and measured after 10 mins by HPLC-MS/MS analysis | Homo sapiens | 1 | ALA4342468 | microsome format | Scientific Literature | |
17. | ALA4344589 | B | Agonist activity at human PPARalpha expressed in nonhuman mammalian cells assessed as maximal increase in receptor transcriptional activity incubated for 22 to 24 hrs by luciferase reporter gene assay relative to GW590735 | Homo sapiens | 52 | ALA4342468 | cell-based format | Scientific Literature | |
18. | ALA4344590 | B | Ratio of agonist activity at human PPARalpha expressed in nonhuman mammalian cells assessed as increase in receptor transcriptional activity by measuring relative light units at 5 uM to agonist activity at human PPARalpha expressed in nonhuman mammalian cells assessed as increase in receptor transcriptional activity by measuring relative light units at 50 uM incubated for 22 to 24 hrs by luciferase reporter gene assay | Homo sapiens | 52 | ALA4342468 | cell-based format | Scientific Literature |