# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4378263 | B | Inhibition of biotinylated DCN1 (unknown origin) - AlexaFluor488 labelled UBE2M (unknown origin) protein-protein interaction after 1 hr by TR-FRET assay | Homo sapiens | 34 | ALA4376818 | protein format | Scientific Literature | |
2. | ALA4378264 | A | Cytotoxicity against human BJ cells assessed as cell growth inhibition after 72 hrs by Celltiter-Glo assay | Homo sapiens | 10 | ALA4376818 | cell-based format | Scientific Literature | |
3. | ALA4378265 | A | Cytotoxicity against human HCC95 cells overexpressing DCN1 assessed as cell growth inhibition after 72 hrs by Celltiter-Glo assay | Homo sapiens | 10 | ALA4376818 | cell-based format | Scientific Literature | |
4. | ALA4378266 | A | Half life in mouse microsomes at 0.8 uM | Mus musculus | 10 | ALA4376818 | microsome format | Scientific Literature | |
5. | ALA4378267 | A | Intrinsic clearance in mouse microsomes at 0.8 uM | Mus musculus | 8 | ALA4376818 | microsome format | Scientific Literature | |
6. | ALA4378268 | P | Kinetic aqueous solubility of compound at pH 7.4 | 10 | ALA4376818 | small-molecule physicochemical format | Scientific Literature | ||
7. | ALA4378269 | B | Inhibition of DCN1 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 10 uM after 10 sec by pulse-chase assay | Homo sapiens | 1 | ALA4376818 | single protein format | Scientific Literature | |
8. | ALA4378270 | B | Inhibition of DCN2 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 10 uM after 10 sec by pulse-chase assay | Homo sapiens | 1 | ALA4376818 | single protein format | Scientific Literature | |
9. | ALA4378271 | B | Inhibition of DCN3 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 120 uM after 10 sec by pulse-chase assay | Homo sapiens | 1 | ALA4376818 | single protein format | Scientific Literature | |
10. | ALA4378272 | B | Inhibition of DCN4 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 60 uM after 10 sec by pulse-chase assay | Homo sapiens | 1 | ALA4376818 | single protein format | Scientific Literature | |
11. | ALA4378273 | B | Inhibition of DCN5 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 60 uM after 10 sec by pulse-chase assay | Homo sapiens | 1 | ALA4376818 | single protein format | Scientific Literature | |
12. | ALA4378274 | B | Binding affinity to DCN1 in human HCC95 cells assessed as increase in thermal stability of DCN1 at 10 uM incubated for 1 hrs at 52 degC by cellular thermal shift assay | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
13. | ALA4378275 | B | Inhibition of DCN1 in human HCC95 cells assessed as decrease in cullin1 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
14. | ALA4378276 | B | Inhibition of DCN1 in human HCC95 cells assessed as decrease in cullin3 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
15. | ALA4378277 | B | Inhibition of DCN1 in human HCC95 cells assessed as decrease in cullin4 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
16. | ALA4378278 | B | Inhibition of DCN1 in human CAL33 cells assessed as decrease in cullin1 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
17. | ALA4378279 | B | Inhibition of DCN1 in human CAL33 cells assessed as decrease in cullin3 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
18. | ALA4378280 | B | Inhibition of DCN1 in human CAL33 cells assessed as decrease in cullin4 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
19. | ALA4378281 | B | Inhibition of DCN1 in human HCC95 cells assessed as decrease in cullin2 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature | |
20. | ALA4378282 | B | Inhibition of DCN1 in human HCC95 cells assessed as decrease in cullin5 neddylation at 10 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4376818 | cell-based format | Scientific Literature |