# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4422542 | Binding | Inhibition of GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli pre-incubated for 10 mins followed by OMFP substrate addition and further incubated for 30 mins by fluorescence based assay | Homo sapiens | 20 | ALA4421733 | assay format | Patent Bioactivity Data | |
2. | ALA4422554 | Binding | Binding affinity to GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli assessed as induction of protein destabilization by measuring reduction in melting temperature in presence of 5 uM Mg2+ ions by thermal shift assay | Homo sapiens | 8 | ALA4421733 | assay format | Patent Bioactivity Data | |
3. | ALA4422555 | Binding | Binding affinity to GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli assessed as induction of protein stabilization by measuring melting temperature in presence of EDTA by thermal shift assay | Homo sapiens | 8 | ALA4421733 | assay format | Patent Bioactivity Data |