#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4431994	Binding	Inhibition of Trypanosoma brucei PTR1 using H2B as substrate incubated for 10 mins followed by addition of NADPH measured for 10 to 50 mins	Trypanosoma brucei	7	ALA4431321	assay format	Scientific Literature
2.	ALA4431995	Binding	Inhibition of human DHFR using folic aid as substrate by spectrophotometric analysis	Homo sapiens	1	ALA4431321	single protein format	Scientific Literature
3.	ALA4431996	Functional	Antitrypanosomal activity against Trypanosoma brucei brucei Lister 427 bloodstream forms after 72 hrs by resazurin-based assay	Trypanosoma brucei brucei	50	ALA4431321	organism-based format	Scientific Literature
4.	ALA4431998	Binding	Selectivity index, ratio of IC50 for human DHFR to IC50 for Trypanosoma brucei PTR1		2	ALA4431321	assay format	Scientific Literature
5.	ALA4431999	Binding	Inhibition of Trypanosoma brucei PTR1 at 50 uM using H2B as substrate incubated for 10 mins followed by addition of NADPH measured for 10 to 50 mins	Trypanosoma brucei	10	ALA4431321	assay format	Scientific Literature
6.	ALA4432000	Binding	Inhibition of Leishmania mexicana PTR1 at 50 uM using H2B as substrate incubated for 10 mins followed by addition of NADPH measured for 10 to 50 mins	Leishmania mexicana	4	ALA4431321	assay format	Scientific Literature
7.	ALA4432001	Binding	Inhibition of Leishmania mexicana DHFR at 50 uM using folic aid as substrate by spectrophotometric analysis	Leishmania mexicana	8	ALA4431321	assay format	Scientific Literature
8.	ALA4432002	Binding	Inhibition of Trypanosoma brucei DHFR at 50 uM using folic aid as substrate by spectrophotometric analysis	Trypanosoma brucei	8	ALA4431321	assay format	Scientific Literature
9.	ALA4432004	Binding	Inhibition of human thymidylate synthase at 50 uM using folic aid as substrate by spectrophotometric analysis	Homo sapiens	8	ALA4431321	single protein format	Scientific Literature
10.	ALA4432005	Binding	Inhibition of human ERG in membranes incubated for 2 hrs by fluorescence polarization assay	Homo sapiens	1	ALA4431321	assay format	Scientific Literature
11.	ALA4432006	Toxicity	Mitochondrial toxicity in human 786-O cells incubated for 6 hrs by mitotracker red staining based-uptake method	Homo sapiens	16	ALA4431321	cell-based format	Scientific Literature
12.	ALA4432007	ADME	Cytotoxicity against human A549 cells at 100 uM after 48 hrs by cell titer glo assay	Homo sapiens	16	ALA4431321	cell-based format	Scientific Literature
13.	ALA4432008	ADME	Cytotoxicity against human WI38 cells at 100 uM after 48 hrs by cell titer glo assay	Homo sapiens	16	ALA4431321	cell-based format	Scientific Literature
14.	ALA4432010	Physicochemical	Solubility of compound in PBS incubated for 18 hrs by UV-visible spectroscopic analysis		1	ALA4431321	small-molecule physicochemical format	Scientific Literature
15.	ALA4432011	ADME	Half life BALB/c mouse blood at 1 mg/kg, iv administered as single dose by HPLC analysis	Mus musculus	1	ALA4431321	organism-based format	Scientific Literature
16.	ALA4432012	ADME	Drug uptake in BALB/c mouse blood at 1 mg/kg, iv administered as single dose after 30 mins by HPLC analysis	Mus musculus	1	ALA4431321	organism-based format	Scientific Literature
17.	ALA4432013	ADME	Drug uptake in BALB/c mouse plasma at 1 mg/kg, iv administered as single dose through encapsulation in PLGA nanoparticles by HPLC analysis	Mus musculus	1	ALA4431321	organism-based format	Scientific Literature
18.	ALA4432014	ADME	Drug uptake in BALB/c mouse plasma at 1 mg/kg, po administered as single dose through encapsulation in PLGA nanoparticles by HPLC analysis	Mus musculus	1	ALA4431321	organism-based format	Scientific Literature
19.	ALA4432015	Functional	Antitrypanosomal activity against Trypanosoma brucei brucei Lister 427 bloodstream forms at 10 uM after 72 hrs by resazurin-based assay	Trypanosoma brucei brucei	13	ALA4431321	organism-based format	Scientific Literature
20.	ALA4432019	ADME	Cytotoxicity against human THP1 cells after 72 hrs by MTT assay	Homo sapiens	16	ALA4431321	cell-based format	Scientific Literature