#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4431994	B	Inhibition of Trypanosoma brucei PTR1 using H2B as substrate incubated for 10 mins followed by addition of NADPH measured for 10 to 50 mins	Trypanosoma brucei	7		assay format	Scientific Literature
2.	ALA4431995	B	Inhibition of human DHFR using folic aid as substrate by spectrophotometric analysis	Homo sapiens	1		single protein format	Scientific Literature
3.	ALA4431996	F	Antitrypanosomal activity against Trypanosoma brucei brucei Lister 427 bloodstream forms after 72 hrs by resazurin-based assay	Trypanosoma brucei brucei	50		organism-based format	Scientific Literature
4.	ALA4431998	B	Selectivity index, ratio of IC50 for human DHFR to IC50 for Trypanosoma brucei PTR1		2		assay format	Scientific Literature
5.	ALA4431999	B	Inhibition of Trypanosoma brucei PTR1 at 50 uM using H2B as substrate incubated for 10 mins followed by addition of NADPH measured for 10 to 50 mins	Trypanosoma brucei	10		assay format	Scientific Literature
6.	ALA4432000	B	Inhibition of Leishmania mexicana PTR1 at 50 uM using H2B as substrate incubated for 10 mins followed by addition of NADPH measured for 10 to 50 mins	Leishmania mexicana	4		assay format	Scientific Literature
7.	ALA4432001	B	Inhibition of Leishmania mexicana DHFR at 50 uM using folic aid as substrate by spectrophotometric analysis	Leishmania mexicana	8		assay format	Scientific Literature
8.	ALA4432002	B	Inhibition of Trypanosoma brucei DHFR at 50 uM using folic aid as substrate by spectrophotometric analysis	Trypanosoma brucei	8		assay format	Scientific Literature
9.	ALA4432004	B	Inhibition of human thymidylate synthase at 50 uM using folic aid as substrate by spectrophotometric analysis	Homo sapiens	8		single protein format	Scientific Literature
10.	ALA4432005	B	Inhibition of human ERG in membranes incubated for 2 hrs by fluorescence polarization assay	Homo sapiens	1		assay format	Scientific Literature
11.	ALA4432006	T	Mitochondrial toxicity in human 786-O cells incubated for 6 hrs by mitotracker red staining based-uptake method	Homo sapiens	16		cell-based format	Scientific Literature
12.	ALA4432007	A	Cytotoxicity against human A549 cells at 100 uM after 48 hrs by cell titer glo assay	Homo sapiens	16		cell-based format	Scientific Literature
13.	ALA4432008	A	Cytotoxicity against human WI38 cells at 100 uM after 48 hrs by cell titer glo assay	Homo sapiens	16		cell-based format	Scientific Literature
14.	ALA4432010	P	Solubility of compound in PBS incubated for 18 hrs by UV-visible spectroscopic analysis		1		small-molecule physicochemical format	Scientific Literature
15.	ALA4432011	A	Half life BALB/c mouse blood at 1 mg/kg, iv administered as single dose by HPLC analysis	Mus musculus	1		organism-based format	Scientific Literature
16.	ALA4432012	A	Drug uptake in BALB/c mouse blood at 1 mg/kg, iv administered as single dose after 30 mins by HPLC analysis	Mus musculus	1		organism-based format	Scientific Literature
17.	ALA4432013	A	Drug uptake in BALB/c mouse plasma at 1 mg/kg, iv administered as single dose through encapsulation in PLGA nanoparticles by HPLC analysis	Mus musculus	1		organism-based format	Scientific Literature
18.	ALA4432014	A	Drug uptake in BALB/c mouse plasma at 1 mg/kg, po administered as single dose through encapsulation in PLGA nanoparticles by HPLC analysis	Mus musculus	1		organism-based format	Scientific Literature
19.	ALA4432015	F	Antitrypanosomal activity against Trypanosoma brucei brucei Lister 427 bloodstream forms at 10 uM after 72 hrs by resazurin-based assay	Trypanosoma brucei brucei	13		organism-based format	Scientific Literature
20.	ALA4432019	A	Cytotoxicity against human THP1 cells after 72 hrs by MTT assay	Homo sapiens	16		cell-based format	Scientific Literature