| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4417199 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring p-nitroanilide release using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay | Homo sapiens | 9 | ALA4414660 | cell-based format | Scientific Literature | |
| 2. | ALA4417201 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat for p-nitroanilide release at 0.1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.8 +/- 0.03 /s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 3. | ALA4417202 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat for p-nitroanilide release at 0.5 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.8 +/- 0.03 /s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 4. | ALA4417203 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat for p-nitroanilide release at 1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.8 +/- 0.03 /s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 5. | ALA4417204 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat for p-nitroanilide release at 5 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.8 +/- 0.03 /s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 6. | ALA4417205 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat for p-nitroanilide release at 10 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.8 +/- 0.03 /s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 7. | ALA4417206 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Km for p-nitroanilide release at 0.1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.59 +/- 0.09 mM) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 8. | ALA4417207 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Km for p-nitroanilide release at 0.5 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.59 +/- 0.09 mM) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 9. | ALA4417208 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Km for p-nitroanilide release at 1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.59 +/- 0.09 mM) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 10. | ALA4417209 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Km for p-nitroanilide release at 5 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.59 +/- 0.09 mM) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 11. | ALA4417210 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Km for p-nitroanilide release at 10 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.59 +/- 0.09 mM) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 12. | ALA4417211 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat/Km ratio for p-nitroanilide release at 0.1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.13 /mM/s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 13. | ALA4417212 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat/Km ratio for p-nitroanilide release at 0.5 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.13 /mM/s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 14. | ALA4417213 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat/Km ratio for p-nitroanilide release at 1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.13 /mM/s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 15. | ALA4417214 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat/Km ratio for p-nitroanilide release at 5 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.13 /mM/s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 16. | ALA4417215 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat/Km ratio for p-nitroanilide release at 10 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.13 /mM/s) | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 17. | ALA4417216 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring reciprocal allosteric coupling constant for hyperbolic mixed predominantly catalytic activation using L-Alanine-p-nitroanilide incubated for 10 mins by p-nitroanilide release detection based UV plate reader based assay | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 18. | ALA4417217 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring factor by which activator affects catalytic constant for hyperbolic mixed predominantly catalytic activation using L-Alanine-p-nitroanilide incubated for 10 mins by p-nitroanilide release detection based UV plate reader based assay | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature | |
| 19. | ALA4417218 | B | Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring dissociation constant (KX) for hyperbolic mixed predominantly catalytic activation using L-Alanine-p-nitroanilide incubated for 10 mins by p-nitroanilide release detection based UV plate reader based assay | Homo sapiens | 5 | ALA4414660 | cell-based format | Scientific Literature |
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