Antiviral activity against HIV-1 NL4-3 infected in human U87 cells expressing CD4.CXCR4 assessed as inhibition of p24 production at 10 uM measured after 4 days by p24 ELISA
Antiviral activity against HIV-1 NL4-3 infected in human U87 cells expressing CD4.CXCR4 assessed as inhibition of p24 production measured after 4 days by p24 ELISA
Antiviral activity against HIV-1 NL4-3 infected in human U87 cells expressing CD4.CXCR4 assessed as inhibition of p24 production at 100 uM measured after 4 days by p24 ELISA
Antiviral activity against HIV-1 NL4-3 infected in human U87 cells expressing CD4.CXCR4 assessed as suppression of p24 production at 100 uM measured after 4 days by p24 ELISA
Binding affinity to HIV-1 RRE 2B RNA assessed as protection against RNase V1-mediated RRE 2B cleavage at G17 and C18 preincubated for 4 hrs followed by addition of RNase V1 measured after 10 mins by nuclease protection assay
Binding affinity to HIV-1 RRE 2B RNA assessed as protection against RNase A-mediated RRE 2B cleavage at C21 and U24 preincubated for 4 hrs followed by addition of RNase A measured up to 20 uM after 1 hr by nuclease protection assay
Binding affinity to HIV-1 RRE 2B RNA assessed as protection against RNase T1-mediated RRE 2B cleavage preincubated for 4 hrs followed by addition of RNase T1 measured up to 20 uM after 1 hr by nuclease protection assay
Binding affinity to HIV-1 RRE 2B RNA assessed as protection against RNase A-mediated RRE 2B cleavage at U9 and G34 preincubated for 4 hrs followed by addition of RNase A at 20 uM measured after 1 hr by nuclease protection assay
Binding affinity to HIV-1 RRE 2B RNA assessed as protection against RNase T1-mediated RRE 2B cleavage at G10 and G11 preincubated for 4 hrs followed by addition of RNase T1 at 20 uM measured after 1 hr by nuclease protection assay
Binding affinity to HIV-1 RRE 2B RNA assessed as protection against RNase A-mediated RRE 2B cleavage at C33 of loop B preincubated for 4 hrs followed by addition of RNase A at 20 uM measured after 1 hr by nuclease protection assay