# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4307649 | B | Binding affinity to recombinant N-terminal His-tagged VRK2 kinase domain (unknown origin) (14 to 335 residues) expressed in Escherichia coli BL 21 DE3-R3 cells assessed as change in melting temperature at 10 uM by thermal shift assay using differential scanning fluorimetry | Homo sapiens | 5 | ALA4304810 | cell-based format | Scientific Literature | |
2. | ALA4307650 | B | Binding affinity to recombinant full length N-terminal His-tagged VRK1 (unknown origin) (1 to 396 residues) expressed in Escherichia coli BL 21 DE3-R3 cells assessed as change in melting temperature at 10 uM by thermal shift assay using differential scanning fluorimetry | Homo sapiens | 8 | ALA4304810 | cell-based format | Scientific Literature | |
3. | ALA4307651 | B | Inhibition of full length wild-type VRK1 (unknown origin) expressed in insect cells using ULight-Histone 3-Thr3 peptide as substrate incubated for 30 mins followed by substrate addition further incubated for 75 mins by fluorescence based assay | Homo sapiens | 5 | ALA4304810 | cell-based format | Scientific Literature | |
4. | ALA4307652 | B | Binding affinity to recombinant N-terminal His-tagged VRK2 kinase domain (unknown origin) (14 to 335 residues) expressed in Escherichia coli BL 21 DE3-R3 cells assessed as dissociation constant at by isothermal titration method | Homo sapiens | 1 | ALA4304810 | cell-based format | Scientific Literature | |
5. | ALA4307654 | A | Cytotoxicity against human HeLa cells assessed as reduction in cell viability at 3.2 uM incubated for 24 hrs by MTT assay | Homo sapiens | 2 | ALA4304810 | cell-based format | Scientific Literature | |
6. | ALA4307655 | A | Cytotoxicity against human HeLa cells assessed as cell viability at 12.5 uM incubated for 24 hrs by MTT assay | Homo sapiens | 1 | ALA4304810 | cell-based format | Scientific Literature |