| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4395087 | A | AUClast in BALB/c mouse at 10 mg/kg, iv measured for 24 hrs post dose by LC-MS/MS analysis | Mus musculus | 1 | ALA4393686 | organism-based format | Scientific Literature | |
| 2. | ALA4395088 | A | AUC (0 to infinity) in BALB/c mouse at 10 mg/kg, iv measured for 24 hrs post dose by LC-MS/MS analysis | Mus musculus | 1 | ALA4393686 | organism-based format | Scientific Literature | |
| 3. | ALA4395090 | B | Inhibition of recombinant His-tagged human PDE4B expressed in Sf9 cells using cAMP as substrate preincubated with enzyme for 15 mins followed by substrate addition and incubated for 1 hr by PDELight HTS cAMP phosphodiesterase Kit based luminometry | Homo sapiens | 11 | ALA4393686 | cell-based format | Scientific Literature | |
| 4. | ALA4395091 | B | Inhibition of human recombinant His-tagged PDE4B expressed in Sf9 cells using cAMP as substrate at 10 uM preincubated with enzyme for 15 mins followed by substrate addition and incubated for 1 hr by PDELight HTS cAMP phosphodiesterase Kit based luminometry relative to control | Homo sapiens | 10 | ALA4393686 | cell-based format | Scientific Literature | |
| 5. | ALA4395092 | A | Inhibition of PDE4D2 (unknown origin) using cAMP as substrate preincubated with enzyme for 15 mins followed by substrate addition and incubated for 1 hr by PDELight HTS cAMP phosphodiesterase Kit based luminometry | Homo sapiens | 6 | ALA4393686 | single protein format | Scientific Literature | |
| 6. | ALA4395093 | U | Selectivity index, ratio of IC50 for PDE4D2 (unknown origin) to IC50 for recombinant His-tagged human PDE4B expressed in sf9 cells using cAMP as substrate by PDELight HTS cAMP phosphodiesterase Kit based luminometry | Homo sapiens | 5 | ALA4393686 | cell-based format | Scientific Literature | |
| 7. | ALA4395094 | A | Inhibition of PDE4C (unknown origin) at 100 uM using cAMP as substrate preincubated with enzyme for 15 mins followed by substrate addition and incubated for 1 hr by PDELight HTS cAMP phosphodiesterase Kit based luminometry relative to control | Homo sapiens | 2 | ALA4393686 | single protein format | Scientific Literature | |
| 8. | ALA4395096 | B | Selectivity for recombinant His-tagged human PDE4B over PDE4C (unknown origin) | Homo sapiens | 2 | ALA4393686 | assay format | Scientific Literature | |
| 9. | ALA4395097 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS expression preincubated for 30 mins followed by LPS stimulation and incubated for 10 hrs by immunoblotting analysis | Mus musculus | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 10. | ALA4395098 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha mRNA expression preincubated for 30 mins followed by LPS stimulation and incubated for 10 hrs by qPCR analysis | Mus musculus | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 11. | ALA4395099 | B | Inhibition of recombinant N-terminal GST-tagged full-length human PDE1A1 expressed in Sf9 cells using FAM-cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 12. | ALA4395100 | B | Inhibition of recombinant N-terminal GST tagged full length human PDE1B expressed in Sf9 cells using FAM-cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 13. | ALA4395101 | B | Inhibition of recombinant N-terminal GST tagged human PDE1C (2 to 634 aa) expressed in Sf9 cells at 10 uM using FAM-cAMP as substrate incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 14. | ALA4395102 | B | Inhibition of recombinant N-terminal-GST-Th-tagged full length human PDE2A1 expressed in Sf9 cells using FAM-cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 15. | ALA4395103 | B | Inhibition of recombinant PDE3A (unknown origin) using FAM-cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | single protein format | Scientific Literature | |
| 16. | ALA4395104 | B | Inhibition of recombinant N-terminal GST-tagged human PDE3B (592-end aa) expressed in Sf9 cells using FAM-cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 17. | ALA4395105 | B | Inhibition of recombinant N-terminal GST-tagged full length human PDE4A1A expressed in Sf9 cells using cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 18. | ALA4395106 | B | Inhibition of recombinant N-terminal GST tagged human PDE4A4B (2-end aa) expressed in Sf9 cells using FAM-cAMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 19. | ALA4395107 | B | Inhibition of recombinant N-terminal GST-tagged full length human PDE5A1 expressed in Sf9 cells using FAM-cGMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature | |
| 20. | ALA4395108 | B | Inhibition of recombinant N-terminal GST-tagged full length human PDE6C expressed in Sf9 cells using FAM-cGMP as substrate at 10 uM incubated for 60 mins by fluorescence plate reader analysis | Homo sapiens | 1 | ALA4393686 | cell-based format | Scientific Literature |
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