# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4417104 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as release of PPi using GPP and IPP as substrate preincubated for 30 mins in absence of catalase followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 2 | ALA4414654 | assay format | Scientific Literature | |
2. | ALA4417105 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as release of PPi using GPP and IPP as substrate preincubated for 30 mins in presence of catalase followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 2 | ALA4414654 | assay format | Scientific Literature | |
3. | ALA4417106 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged GGPPS expressed in Escherichia coli BL21 (DE3) using FPP and [14C] IPP as substrate preincubated for 15 mins with FPP followed by [14C] IPP addition and further incubated for 20 mins by scintillation counting method | Homo sapiens | 57 | ALA4414654 | assay format | Scientific Literature | |
4. | ALA4417107 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 58 | ALA4414654 | assay format | Scientific Literature | |
5. | ALA4417108 | B | Selectivity index, ratio of IC50 for human N-terminal TEV protease cleavage site-fused-His6-tagged GGPPS expressed in Escherichia coli BL21 (DE3) to IC50 for human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 16 | ALA4414654 | assay format | Scientific Literature | |
6. | ALA4417110 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition in presence of 0.01 % Triton X-100 by continuous spectrophotometric assay | Homo sapiens | 14 | ALA4414654 | assay format | Scientific Literature | |
7. | ALA4417111 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition in absence of 0.01 % Triton X-100 by continuous spectrophotometric assay | Homo sapiens | 14 | ALA4414654 | assay format | Scientific Literature | |
8. | ALA4417124 | F | Cytotoxicity against human PANC1 cells assessed as decrease in cell growth measured after 72 hrs by MTT assay | Homo sapiens | 32 | ALA4414654 | cell-based format | Scientific Literature | |
9. | ALA4417125 | F | Cytotoxicity against human MIAPaCa2 cells assessed as decrease in cell growth measured after 72 hrs by MTT assay | Homo sapiens | 32 | ALA4414654 | cell-based format | Scientific Literature | |
10. | ALA4417147 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition in presence of Mg2+ by continuous spectrophotometric assay | Homo sapiens | 2 | ALA4414654 | assay format | Scientific Literature |