#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4432140	A	Cytotoxicity in RFC-null Chinese hamster R2 cells assessed as reduction in cell viability measured after 96 hrs by Cell-Titer Blue fluorescence analysis	Cricetulus griseus	10	ALA4431326	cell-based format	Scientific Literature
2.	ALA4432141	B	Inhibition of human RFC expressed in Chinese hamster PC43-10 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
3.	ALA4432142	B	Inhibition of human FR-alpha receptor expressed in Chinese hamster RT16 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
4.	ALA4432143	B	Inhibition of human FR-beta receptor expressed in Chinese hamster D4 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
5.	ALA4432144	B	Inhibition of human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as antiproliferative activity measured as reduction in cell viability after 96 hrs by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
6.	ALA4432145	A	Cytotoxicity in PCFT-null Chinese hamster R2 cells assessed as reduction in cell viability measured after 96 hrs by Cell-Titer Blue fluorescence analysis	Cricetulus griseus	6	ALA4431326	cell-based format	Scientific Literature
7.	ALA4432146	F	Antiproliferative activity against human KB cells expressing human RFC/FR-alpha/PCFT assessed as reduction in cell viability measured after 96 hrs by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
8.	ALA4432147	F	Antiproliferative activity against human KB cells expressing human RFC/FR-alpha/PCFT assessed as reduction in cell viability measured after 96 hrs in presence of folic acid by Cell-Titer Blue fluorescence analysis	Homo sapiens	7	ALA4431326	cell-based format	Scientific Literature
9.	ALA4432148	B	Inhibition of GARFTase/AICARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as cell growth inhibition measured after 96 hrs in presence of 60 uM adenosine by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
10.	ALA4432149	B	Inhibition of GARFTase/AICARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as cell growth inhibition measured after 96 hrs in presence of 10 uM thymidine by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
11.	ALA4432150	B	Inhibition of AICARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as cell growth inhibition measured after 96 hrs in presence of 320 uM AICA by Cell-Titer Blue fluorescence analysis	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
12.	ALA4432151	B	Displacement of [3H]folic acid from human FR-alpha receptor expressed in Chinese hamster RT16 cells measured after 15 mins relative to folic acid	Homo sapiens	6	ALA4431326	cell-based format	Scientific Literature
13.	ALA4432154	B	Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at 10 uM and pH 5.5 measured after 5 mins	Homo sapiens	7	ALA4431326	cell-based format	Scientific Literature
14.	ALA4432155	B	Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at 10 uM and pH 6.8 measured after 5 mins	Homo sapiens	7	ALA4431326	cell-based format	Scientific Literature
15.	ALA4432156	B	Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at 1 uM and pH 5.5 measured after 5 mins	Homo sapiens	7	ALA4431326	cell-based format	Scientific Literature
16.	ALA4432157	B	Inhibition of [3H]MTX uptake at human PCFT expressed in Chinese hamster R2/PCFT4 cells at pH 5.5 measured after 5 mins by Dixon plot analysis	Homo sapiens	8	ALA4431326	cell-based format	Scientific Literature
17.	ALA4432158	B	Inhibition of GARFTase in human KB cells expressing RFC/FR-alpha/PCFT assessed as decrease in incorporation of [14C(U)]-glycine into [14C]formyl GAR incubated for 1 hr followed by addition of [14C(U)]-glycine measured after 16 hrs in presence of azaserine	Homo sapiens	7	ALA4431326	cell-based format	Scientific Literature
18.	ALA4432159	B	Inhibition of recombinant N-terminal 6His-tagged human GARFTase assessed as formation of 5,8-dideazafolate from 10-formyl-5,8-dideazafolic acid measured at every 15 seconds over 20 min by spectrophotometric analysis	Homo sapiens	9	ALA4431326	single protein format	Scientific Literature
19.	ALA4432160	B	Potency index, ratio of PMX Ki to test compound Ki for recombinant N-terminal 6His-tagged human GARFTase	Homo sapiens	8	ALA4431326	single protein format	Scientific Literature