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# Aladdin ID Assay Type Description Organism Compounds Reference BAO Format Source 1. ALA4422045 Binding Inhibition of N-terminal His6-Smt3-fusion tagged human Usp9x (1553 to 1960 amino acids) expressed in Escherichia coli pre-incubated for 30 mins before Ub-AMC substrate addition by fluorescence based assay Homo sapiens 10 ALA4421725 assay format Patent Bioactivity Data 2. ALA4422046 Binding Inhibition of Usp9x in human Z138 cells at 1.25 to 5 uM incubated for 4 hrs by immunoblotting analysis Homo sapiens 3 ALA4421725 cell-based format Patent Bioactivity Data 3. ALA4422047 Binding Inhibition of Usp9x in human Z138 cells assessed as reduction in Mcl1 protein levels at 1.25 to 5 uM incubated for 4 hrs by immunoblotting analysis Homo sapiens 3 ALA4421725 cell-based format Patent Bioactivity Data 4. ALA4422056 ADME Half life in orally dosed Balb/c mouse by LC-MS analysis Mus musculus 2 ALA4421725 organism-based format Patent Bioactivity Data 5. ALA4422057 ADME Half life in ip dosed Balb/c mouse by LC-MS analysis Mus musculus 2 ALA4421725 organism-based format Patent Bioactivity Data 6. ALA4422058 ADME Plasma concentration in Balb/c mouse at 5 mg/kg, iv after 0.5 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 7. ALA4422059 ADME Plasma concentration in Balb/c mouse at 5 mg/kg, iv after 2 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 8. ALA4422060 ADME Plasma concentration in Balb/c mouse at 5 mg/kg, iv after 4 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 9. ALA4422061 ADME Plasma concentration in Balb/c mouse at 5 mg/kg, iv after 7 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 10. ALA4422062 ADME AUClast in Balb/c mouse at 5 mg/kg, iv by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 11. ALA4422063 ADME Plasma concentration in Balb/c mouse at 10 mg/kg, po after 0.5 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 12. ALA4422064 ADME Plasma concentration in Balb/c mouse at 10 mg/kg, po after 2 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 13. ALA4422065 ADME Plasma concentration in Balb/c mouse at 10 mg/kg, po after 4 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 14. ALA4422066 ADME Plasma concentration in Balb/c mouse at 10 mg/kg, po after 7 hrs by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data 15. ALA4422067 ADME AUClast in Balb/c mouse at 10 mg/kg, po by LC-MS analysis Mus musculus 1 ALA4421725 organism-based format Patent Bioactivity Data