| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4359450 | B | Inhibition of recombinant human GST-tagged RET cytoplasmic domain (658 to 1114 residues) expressed in baculovirus expression system using 5'FAM-EPLYWSFPA as substrate preincubated for 60 mins followed by substrate addition by microfluidic assay | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 2. | ALA4359451 | B | Inhibition of recombinant human N-terminal GST/His-tagged RET V804M mutant (658 to 1114 residues) expressed in Sf9 insect cells using 5'FAM-EPLYWSFPA as substrate preincubated for 60 mins followed by substrate addition by microfluidic assay | Homo sapiens | 1 | ALA4354879 | cell-based format | Scientific Literature | |
| 3. | ALA4359452 | B | Inhibition of recombinant human His-tagged VEGFR2 cytoplasmic domain (789 to 1356 residues) expressed in baculovirus expression system using peptide substrate preincubated for 60 mins followed by substrate addition by microfluidic assay | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 4. | ALA4359453 | B | Binding affinity to recombinant phosphorylated RET kinase domain (705 to 1013 residues) (unknown origin) expressed in baculovirus infected Sf9 insect cells assessed as change in melting temperature at 40 uM measured over 240 mins by sypro orange dye based thermal shift assay | Homo sapiens | 1 | ALA4354879 | cell-based format | Scientific Literature | |
| 5. | ALA4359454 | B | Binding affinity to recombinant RET V804M mutant (unknown origin) expressed in baculovirus infected Sf9 insect cells assessed as change in melting temperature at 40 uM by sypro orange dye based thermal shift assay | Homo sapiens | 2 | ALA4354879 | cell-based format | Scientific Literature | |
| 6. | ALA4359456 | B | Binding affinity to wild-type human partial length CSF1R (I564 to S939 residues) expressed in bacterial expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 7. | ALA4359457 | B | Binding affinity to wild-type human partial length FRK (A215 to F503 residues) expressed in bacterial expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 8. | ALA4359458 | B | Binding affinity to wild-type human full length HCK (M22 to P526 residues) expressed in bacterial expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 9. | ALA4359459 | B | Binding affinity to wild-type human partial length LYN (C219 to Q511 residues) expressed in bacterial expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 10. | ALA4359460 | B | Binding affinity to wild-type human partial length MKNK2 (G63 to S388 residues) expressed in mammalian expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 11. | ALA4359461 | B | Binding affinity to wild-type human partial length TRKA (G475 to G790 residues) expressed in mammalian expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 12. | ALA4359462 | B | Binding affinity to wild-type human partial length TRKC (Q531 to G825 residues) expressed in mammalian expression system at 100 nM by Kinomescan method relative to control | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 13. | ALA4359463 | B | Inhibition of recombinant human His-tagged TRKA cytoplasmic domain (441 to 796 residues) expressed in baculovirus expression system using peptide substrate preincubated for 60 mins followed by substrate addition by microfluidic assay | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 14. | ALA4359464 | B | Inhibition of recombinant human His-tagged CSF1R cytoplasmic domain (538 to 910 residues) expressed in baculovirus expression system using peptide substrate preincubated for 60 mins followed by substrate addition by microfluidic assay | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 15. | ALA4359465 | B | Inhibition of human ERG expressed in HEK293 cells by patch clamp assay | Homo sapiens | 2 | ALA4354879 | cell-based format | Scientific Literature | |
| 16. | ALA4359466 | B | Selectivity ratio of IC50 for human ERG expressed in HEK293 cells to IC50 for recombinant human GST-tagged RET cytoplasmic domain (658 to 1114 residues) expressed in baculovirus expression system | Homo sapiens | 1 | ALA4354879 | cell-based format | Scientific Literature | |
| 17. | ALA4359467 | B | Effect on SHC phosphorylation in RET deficient human Nthy-ori 3-1 cells at 1 to 100 nM measured after 2 hrs by western blot analysis | Homo sapiens | 1 | ALA4354879 | assay format | Scientific Literature | |
| 18. | ALA4359468 | B | Inhibition of RET isoform 9 C634R mutant (unknown origin) expressed in rat Rat1 assessed as reduction in RET phosphorylation at Y905 residue at 0.3 nM measured after 2 hrs by Western blot analysis | Homo sapiens | 1 | ALA4354879 | cell-based format | Scientific Literature | |
| 19. | ALA4359469 | B | Inhibition of RET isoform 9 C634R mutant (unknown origin) expressed in rat Rat1 assessed as reduction in RET phosphorylation at Y1062 residue at 0.3 nM measured after 2 hrs by Western blot analysis | Homo sapiens | 1 | ALA4354879 | cell-based format | Scientific Literature | |
| 20. | ALA4359470 | B | Inhibition of RET isoform 9 C634R mutant (unknown origin) expressed in rat Rat1 assessed as reduction in RET phosphorylation at Y905 residue at 3 nM measured after 2 hrs by Western blot analysis | Homo sapiens | 1 | ALA4354879 | cell-based format | Scientific Literature |
The page will load shortly, Thanks for your patience!