Anti-osteoporosis activity in C57BL/6 mouse bone marrow macrophages assessed as inhibition of M-CSF/RANKL-induced osteoclast differentiation supplemented with fresh medium containing compounds every 2 days for 5 measured post-last dose by TRAP staining based assay
Anti-osteoporosis activity in C57BL/6 mouse bone marrow macrophages assessed as inhibition of M-CSF/RANKL-induced osteoclast differentiation by measuring reduction in TRAcP gene expression at 0.1 uM supplemented with fresh medium containing compounds every 2 days for 5 by trizol-reagent based RT-PCR analysis
Anti-osteoporosis activity in C57BL/6 mouse bone marrow macrophages assessed as inhibition of M-CSF/RANKL-induced osteoclast differentiation by measuring reduction in c-FOS gene expression at 0.1 uM supplemented with fresh medium containing compounds every 2 days for 5 by trizol-reagent based RT-PCR analysis
Anti-osteoporosis activity in C57BL/6 mouse bone marrow macrophages assessed as inhibition of M-CSF/RANKL-induced osteoclast differentiation by measuring reduction in MMP9 gene expression at 0.1 uM supplemented with fresh medium containing compounds every 2 days for 5 by trizol-reagent based RT-PCR analysis
Anti-osteoporosis activity in C57BL/6 mouse bone marrow macrophages assessed as inhibition of M-CSF/RANKL-induced osteoclast differentiation by measuring reduction in NFATc1 gene expression at 0.1 uM supplemented with fresh medium containing compounds every 2 days for 5 by trizol-reagent based RT-PCR analysis
Anti-osteoporosis activity in C57BL/6 mouse bone marrow macrophages assessed as inhibition of M-CSF/RANKL-induced osteoclast differentiation by measuring reduction in hydroxyapatite resorption at 0.01 to 1 uM measured after 48 hrs by microscopic analysis
Osteogenic activity in mouse C3H10T1/2 cells assessed as effect on osteogenic medium-induced osteoblast differentiation by measuring calcium deposition at 0.1 to 10 uM after 14 days by alizarin red dye-based assay
Anti-osteoporosis activity in ovariectomized Sprague-Dawley rat assessed as reduction in femur bone mass loss at 10 mg/kg/day, po for 3 months measured post-last dose by micro-CT analysis
Anti-osteoporosis activity in ovariectomized Sprague-Dawley rat assessed as femur bone mineral density loss at 10 mg/kg/day, po for 3 months measured post-last dose by dual energy X-ray absorptiometry (RVb = 19%)
Anti-osteoporosis activity in ovariectomized Sprague-Dawley rat assessed as reduction in serum CTX1 level at 10 mg/kg/day, po for 3 months measured post-last dose
Anti-osteoporosis activity in ovariectomized Sprague-Dawley rat assessed as reduction in serum osteocalcin level at 10 mg/kg/day, po for 3 months measured post-last dose
Anti-osteoporosis activity in ovariectomized Sprague-Dawley rat assessed as reduction in serum PINP level at 10 mg/kg/day, po for 3 months measured post-last dose
Toxicity in ovariectomized Sprague-Dawley rat assessed as effect on alanine aminotransferase level at 10 mg/kg/day, po for 3 months measured post-last dose
Toxicity in ovariectomized Sprague-Dawley rat assessed as effect on aspartate transaminase level at 10 mg/kg/day, po for 3 months measured post-last dose
Toxicity in ovariectomized Sprague-Dawley rat assessed as effect on alkaline phosphatase level at 10 mg/kg/day, po for 3 months measured post-last dose