| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4346049 | Binding | Inhibition of recombinant human GST-tagged p300 HAT domain (1195 to 1673 residues) expressed in Escherichia coli BL21 codon plus cells using Histone H3 peptide (1 to 21) and [3H]acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 37 | ALA4342520 | cell-based format | Scientific Literature | |
| 2. | ALA4346052 | Binding | Potency index, ratio of IC50 of 4,5-Bis(4-(tert-butyl)phenyl)-N-(piperidin-4-ylmethyl)thiophene-2-carboxamide to IC50 of compund for inhibition of recombinant human GST-tagged p300 HAT domain (1195 to 1673 residues) expressed in Escherichia coli BL21 codon plus cells using Histone H3 (1 to 21) peptide and [3H]acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 3. | ALA4346053 | Binding | Non-competitive inhibition of recombinant human GST-tagged p300 HAT domain (1195 to 1673 residues) expressed in Escherichia coli BL21 codon plus cells using histone H3 (1-21) peptide and increasing concentration of acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 4. | ALA4346054 | Binding | Competitive inhibition of recombinant human GST-tagged p300 HAT domain (1195 to 1673 residues) expressed in Escherichia coli BL21 codon plus cells using 2 to 100 uM histone H3 (1-21) peptide and acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 5. | ALA4346055 | Binding | Inhibition of His6-tagged p300 HAT domain (unknown origin) assessed as disruption of p300 and Histone H4 (1-21) peptide interaction incubated for 1 hr by Alpha assay | Homo sapiens | 3 | ALA4342520 | single protein format | Scientific Literature | |
| 6. | ALA4346056 | Binding | Inhibition of recombinant human GST-tagged CBP HAT domain expressed in Escherichia coli BL21 codon plus cells using Histone H3 peptide (1 to 21) and [3H]acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 7. | ALA4346057 | Binding | Inhibition of recombinant human GST-tagged PCAF expressed in Escherichia coli BL21 codon plus cells using Histone H3 peptide (1 to 21) and [3H]acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 8. | ALA4346058 | Binding | Inhibition of recombinant human GST-tagged Myst3 expressed in Escherichia coli BL21 codon plus cells using Histone H3 peptide (1 to 21) and [3H]acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 9. | ALA4346059 | Binding | Inhibition of LSD1 (unknown origin) at 50 uM | Homo sapiens | 2 | ALA4342520 | single protein format | Scientific Literature | |
| 10. | ALA4346060 | Binding | Inhibition of p300/CBP HAT domain in human Kasumi-1 cells assessed as reduction in H3K9 acetylation incubated for 12 hrs by Western blot analysis | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 11. | ALA4346061 | Binding | Inhibition of p300/CBP HAT domain in human Kasumi-1 cells assessed as reduction in H3K18 acetylation incubated for 12 hrs by Western blot analysis | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 12. | ALA4346062 | Binding | Inhibition of p300/CBP HAT domain in human Kasumi-1 cells assessed as reduction in H3K27 acetylation incubated for 12 hrs by Western blot analysis | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 13. | ALA4346063 | Functional | Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 6 days by Celigo-insitu cell cytometer analysis | Homo sapiens | 4 | ALA4342520 | cell-based format | Scientific Literature | |
| 14. | ALA4346064 | Functional | Antiproliferative activity against human tamoxifen-resistant MCF7 cells assessed as reduction in cell viability incubated for 6 days by Celigo-insitu cell cytometer analysis | Homo sapiens | 4 | ALA4342520 | cell-based format | Scientific Literature | |
| 15. | ALA4346065 | Functional | Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability incubated for 5 days by MTT assay | Homo sapiens | 4 | ALA4342520 | cell-based format | Scientific Literature | |
| 16. | ALA4346066 | Functional | Antiproliferative activity against human MDAPanc28 cells assessed as reduction in cell viability incubated for 5 days by MTT assay | Homo sapiens | 4 | ALA4342520 | cell-based format | Scientific Literature | |
| 17. | ALA4346067 | Functional | Antiproliferative activity against human Kasumi-1 cells assessed as reduction in cell viability incubated for 5 days by XTT assay | Homo sapiens | 4 | ALA4342520 | cell-based format | Scientific Literature | |
| 18. | ALA4346068 | Binding | Inhibition of p300 in human MCF7 cells assessed as estradiol-induced E2F gene expression at 6 uM incubated for 48 hrs by RNA gene sequencing analysis relative to control (Rvb = 7.33 No_unit) | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 19. | ALA4346069 | Binding | Inhibition of p300 in human MCF7 cells assessed as estradiol-induced late estrogen response related gene expression at 6 uM incubated for 48 hrs by RNA gene sequencing analysis relative to control (Rvb = 6.30 No_unit) | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature | |
| 20. | ALA4346070 | Binding | Inhibition of p300 in human MCF7 cells assessed as estradiol-induced G2M checkpoint related gene expression at 6 uM incubated for 48 hrs by RNA gene sequencing analysis relative to control (Rvb = 5.54 No_unit) | Homo sapiens | 1 | ALA4342520 | cell-based format | Scientific Literature |
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