# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4375047 | ADME | Metabolic stability in rat liver microsomes preincubated for 5 mins followed by NADPH addition and measured after 5 to 45 mins by LC/MS analysis | Rattus norvegicus | 1 | ALA4373695 | microsome format | Scientific Literature | |
2. | ALA4375048 | ADME | Inhibition of wild-type EGFR (unknown origin) using Poly(Glu,Tyr) 4:1 as substrate after 60 mins by ELISA | Homo sapiens | 17 | ALA4373695 | single protein format | Scientific Literature | |
3. | ALA4375049 | Binding | Inhibition of human C-terminal His-tagged and N-terminal GST-tagged EGFR L858R/T790M/C797S triple mutant ( 668 to 1210 amino acids) expressed in baculovirus infected Sf9 insect cells using Poly(Glu,Tyr) 4:1 as substrate after 60 mins by ELISA | Homo sapiens | 17 | ALA4373695 | cell-based format | Scientific Literature | |
4. | ALA4375050 | Binding | Inhibition of EGFR L858R/T790M/C797S triple mutant (unknown origin) transfected in mouse BAF3 cells assessed as reduction in EGF-induced EGFR phosphorylation at 0.1 to 3 uM by Western blot analysis | Homo sapiens | 1 | ALA4373695 | cell-based format | Scientific Literature | |
5. | ALA4375051 | ADME | Inhibition of wild-type EGFR (unknown origin) transfected in human A431 cells assessed as reduction in EGF-induced EGFR phosphorylation at 0.1 to 3 uM by Western blot analysis | Homo sapiens | 1 | ALA4373695 | cell-based format | Scientific Literature | |
6. | ALA4375052 | Functional | Antiproliferative activity against mouse BAF3 cells harboring EGFR L858R/T790M/C797S triple mutant after 72 hrs by resazurin dye-based fluorescence assay | Mus musculus | 1 | ALA4373695 | cell-based format | Scientific Literature | |
7. | ALA4375053 | Physicochemical | Aqueous solubility of the compound at 2 mg at pH 7.4 after 24 hrs by LC-MS/MS analysis | 1 | ALA4373695 | small-molecule physicochemical format | Scientific Literature | ||
8. | ALA4375054 | ADME | Permeability of the compound in human Caco2 cells at 10 uM after 120 mins by LC-MS/MS analysis | Homo sapiens | 1 | ALA4373695 | cell-based format | Scientific Literature |