#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4336151	Functional	Induction of NO generation in Leishmania major MRHO/IR/75/ER amastigotes infected in BALB/c mouse macrophage at 0.5 times IC50 to IC50 after 6 hrs by Griess reagent based method	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
2.	ALA4336152	Functional	Antileishmanial activity against Leishmania major MRHO/IR/75/ER promastigotes assessed as reduction in parasite growth after 24 hrs by MTT assay	Leishmania major	7	ALA4334457	organism-based format	Scientific Literature
3.	ALA4336153	Functional	Antileishmanial activity against Leishmania major MRHO/IR/75/ER promastigotes assessed as reduction in parasite growth after 48 hrs by MTT assay	Leishmania major	7	ALA4334457	organism-based format	Scientific Literature
4.	ALA4336154	Functional	Antileishmanial activity against Leishmania major MRHO/IR/75/ER amastigotes infected in BALB/c mouse peritoneal macrophage after 24 hrs by Wright-Giemsa staining based assay	Leishmania major	7	ALA4334457	organism-based format	Scientific Literature
5.	ALA4336155	ADME	Cytotoxicity against mouse peritoneal macrophages assessed as reduction in cell viability after 24 hrs by colorimetric based MTT assay	Mus musculus	7	ALA4334457	cell-based format	Scientific Literature
6.	ALA4336156	Toxicity	Selectivity index, ratio of CC50 for mouse peritoneal macrophages to IC50 for Leishmania major MRHO/IR/75/ER amastigotes infected in BALB/c mouse peritoneal macrophage		7	ALA4334457	assay format	Scientific Literature
7.	ALA4336157	Functional	Induction of ROS generation in Leishmania major MRHO/IR/75/ER promastigotes infected in BALB/c mouse peritoneal macrophage at 0.5 times IC50 to 2 times IC50 after 6 hrs by H2DCFDA staining based fluorescence method	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
8.	ALA4336158	Functional	Induction of free radical generation in promastigote forms of Leishmania major MRHO/IR/75/ER assessed as reduction in antioxidant capacity at IC50 after 24 hrs by FRAP assay	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
9.	ALA4336159	Functional	Induction of free radical generation in promastigote forms of Leishmania major MRHO/IR/75/ER assessed as reduction in GSH level at IC50 after 24 hrs by Ellman's method	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
10.	ALA4336160	Functional	Induction of GPx activity in promastigote forms of Leishmania major MRHO/IR/75/ER at IC50 after 24 hrs	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
11.	ALA4336161	Functional	Induction of ROS generation in amastigote forms of Leishmania major MRHO/IR/75/ER infected in BALB/c mouse macrophage at 0.5 times IC50 to 2 times IC50 after 6 hrs by H2DCFDA staining based fluorescence method	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
12.	ALA4336162	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as increase in caspase-3 activity at IC50 using Ac-DEVD-pNA as substrate preincubated for 24 hrs followed by substrate addition and measured after 1 hr by spectrophotometric method	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
13.	ALA4336163	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as decrease in procaspase-3 level at IC50 by Western blot analysis	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
14.	ALA4336164	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as viable cells at IC50 after 12 hrs by Annexin V-FITC/propidium iodide staining based flow cytometric analysis (Rvb = 92.7%)	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
15.	ALA4336165	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as early apoptotic cells at IC50 after 12 hrs by Annexin V-FITC/propidium iodide staining based flow cytometric analysis (Rvb = 2.59%)	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
16.	ALA4336166	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as late apoptotic cells at IC50 after 12 hrs by Annexin V-FITC/propidium iodide staining based flow cytometric analysis (Rvb = 4.2%)	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
17.	ALA4336167	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as necrotic cells at IC50 after 12 hrs by Annexin V-FITC/propidium iodide staining based flow cytometric analysis (Rvb = 0.548%)	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
18.	ALA4336168	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as viable cells at IC50 after 12 hrs by TUNEL assay (Rvb = 95.7%)	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
19.	ALA4336169	Functional	Induction of apoptosis in Leishmania major MRHO/IR/75/ER promastigotes assessed as apoptotic cells at IC50 after 12 hrs by TUNEL assay (Rvb = 4.31%)	Leishmania major	2	ALA4334457	organism-based format	Scientific Literature
20.	ALA4336170	Functional	Induction of NO generation in amastigote forms of Leishmania major MRHO/IR/75/ER infected in BALB/c mouse macrophage at 2 times IC50 after 6 hrs by Griess reagent based method	Leishmania major	1	ALA4334457	organism-based format	Scientific Literature