#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4324039	Functional	Anticlonogenic activity against human SW620 cells assessed as loss of cancer stem cell stemness by measuring inhibition of colony formation at 10 uM incubated for 24 hrs followed by replating and measured after 7 days by clonogenic assay	Homo sapiens	8	ALA4321838	cell-based format	Scientific Literature
2.	ALA4324040	Functional	Antiinvasive activity in human SW620 organoids transduced with NucLight-Red virus assessed as inhibition of cell invasion at 10 uM incubated for 72 hrs and measured after 72 hrs by matrigel based fluorescence analysis	Homo sapiens	8	ALA4321838	cell-based format	Scientific Literature
3.	ALA4324041	Binding	Inhibition of human topoisomerase 2alpha mediated decatenation at 30 uM using kDNA as susbtrate preincubated for 20 mins followed by substrate and ATP addition and measured after 1 hr by ethidium bromide staining based gel electrophoresis method relative to control	Homo sapiens	30	ALA4321838	single protein format	Scientific Literature
4.	ALA4324042	Binding	Inhibition of human topoisomerase 2alpha mediated decatenation using kDNA as susbtrate preincubated for 20 mins followed by substrate and ATP addition and measured after 1 hr by ethidium bromide staining based gel electrophoresis method	Homo sapiens	30	ALA4321838	single protein format	Scientific Literature
5.	ALA4324043	Binding	Inhibition of topoisomerase 2alpha dependent TCF transcription in human SW620 organoids transduced with TOPflash luminescent reporter after 72 hrs by luciferase based TOPFlash reporter gene assay	Homo sapiens	10	ALA4321838	single protein format	Scientific Literature
6.	ALA4324044	Functional	Cytotoxicity against human SW620 organoids assessed as cell death with loss of membrane integrity after 72 hrs by CellTox Green assay	Homo sapiens	10	ALA4321838	cell-based format	Scientific Literature
7.	ALA4324045	Binding	Inhibition of topoisomerase 2alpha in human SW620 organoids transfected with VimPro-GFP reporter assessed as reversion of epithelial-mesenchymal transition by measuring reduction in vimentin expression after 72 hrs by Hoechst 33342 staining based GFP reporter gene assay	Homo sapiens	10	ALA4321838	assay format	Scientific Literature
8.	ALA4324046	Binding	Inhibition of topoisomerase 2alpha in human SW620 organoids transfected with EcadPro-RFP reporter assessed as reversion of epithelial-mesenchymal transition by measuring increase in E-cadherin expression after 72 hrs by Hoechst 33342 staining based mCherryRFP reporter gene assay	Homo sapiens	10	ALA4321838	assay format	Scientific Literature
9.	ALA4324051	Physicochemical	Aqueous solubility of compound in PBS at 1 mg after 18 hrs by UV based analysis		5	ALA4321838	small-molecule physicochemical format	Scientific Literature
10.	ALA4324052	ADME	Half life in CD-1 mouse microsomes measured up to 1 hr by LC-MS analysis	Mus musculus	5	ALA4321838	microsome format	Scientific Literature
11.	ALA4324053	ADME	Cmax in CD-1 mouse at 50 mg/kg, ip by LC-MS/MS analysis	Mus musculus	5	ALA4321838	organism-based format	Scientific Literature
12.	ALA4324054	ADME	Tmax in CD-1 mouse at 50 mg/kg, ip by LC-MS/MS analysis	Mus musculus	5	ALA4321838	organism-based format	Scientific Literature
13.	ALA4324055	ADME	Apparent volume of drug distribution in CD-1 mouse at 50 mg/kg, ip by LC-MS/MS analysis	Mus musculus	5	ALA4321838	organism-based format	Scientific Literature
14.	ALA4324056	ADME	AUC(0 to t) in CD-1 mouse at 50 mg/kg, ip by LC-MS/MS analysis	Mus musculus	5	ALA4321838	organism-based format	Scientific Literature
15.	ALA4324057	ADME	Clearance in CD-1 mouse at 50 mg/kg, ip by LC-MS/MS analysis	Mus musculus	5	ALA4321838	organism-based format	Scientific Literature
16.	ALA4324058	ADME	Half life of terminal disposition phase in CD-1 mouse at 50 mg/kg, ip by LC-MS/MS analysis	Mus musculus	5	ALA4321838	organism-based format	Scientific Literature
17.	ALA4324060	ADME	Drug metabolism in CD-1 mouse active microsomes assessed as CYP450-mediated oxidative phase 1 metabolite formation measured within 30 mins by LC-MS/MS analysis	Mus musculus	8	ALA4321838	microsome format	Scientific Literature
18.	ALA4324061	ADME	Metabolic stability in CD-1 mouse inactive microsomes incubated for 1 hr by LC-MS/MS analysis	Mus musculus	11	ALA4321838	microsome format	Scientific Literature
19.	ALA4324063	ADME	Drug metabolism in CD-1 mouse active microsomes assessed as metabolite formation at rate of first-order kinetics by LC-MS/MS analysis	Mus musculus	7	ALA4321838	microsome format	Scientific Literature