# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4374800 | B | Inhibition of Mycobacterium tuberculosis LeuRS expressed in Escherichia coli Rosetta (DE3) cells at 100 uM incubated for 30 mins followed by incubation with ATP, leucine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis relative to control | Mycobacterium tuberculosis | 37 | ALA4373683 | cell-based format | Scientific Literature | |
2. | ALA4374801 | B | Inhibition of recombinant Mycobacterium tuberculosis H37Rv C-terminal His6-tagged MetRS expressed in Escherichia coli BL21 (DE3) Star cells at 100 uM incubated for 30 mins followed by incubation with ATP, methionine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis relative to control | Mycobacterium tuberculosis H37Rv | 37 | ALA4373683 | cell-based format | Scientific Literature | |
3. | ALA4374814 | B | Inhibition of yeast inorganic pyrophosphatase assessed as inhibition of BIOMOL-Green signal using inorganic pyrophosphate as substrate at 50 uM incubated for 10 mins in MtbMetRS reaction buffer and measured after 30 mins by BIOMOL-Green reagent based plate reader analysis | Saccharomyces cerevisiae | 10 | ALA4373683 | assay format | Scientific Literature | |
4. | ALA4374815 | U | Inhibition of BIOMOL-Green signal at 50 uM in MtbMetRS reaction buffer in presence of 10 uM phosphate standard measured after 30 mins by plate reader analysis | 10 | ALA4373683 | assay format | Scientific Literature |