# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4427007 | B | Inhibition of human C-terminal HA-tagged DAGLalpha expressed in HEK293F cell membrane fractions using PNP butyrate as substrate by colorimetric surrogate substrate assay | Homo sapiens | 2 | ALA4425142 | cell-based format | Scientific Literature | |
2. | ALA4427008 | B | Inhibition of human C-terminal HA-tagged DAGLalpha expressed in HEK293F cell membrane fractions using DiFMU-octanoate as substrate by fluorogenic surrogate substrate assay | Homo sapiens | 2 | ALA4425142 | cell-based format | Scientific Literature | |
3. | ALA4427009 | B | Inhibition of human GST-tagged DAGLbeta catalytic domain expressed in HEK293F cell membranes using EnzChek as substrate preincubated for 5 mins followed by substrate addition by fluorogenic surrogate substrate assay | Homo sapiens | 2 | ALA4425142 | cell-based format | Scientific Literature | |
4. | ALA4427010 | B | Inhibition of human GST-tagged DAGLalpha expressed in HEK293F cell membranes using 1-stearoyl-2-arachidonoyl-sn-glycerol as substrate preincubated for 20 mins followed by substrate addition by enzyme coupled natural substrate assay | Homo sapiens | 3 | ALA4425142 | cell-based format | Scientific Literature | |
5. | ALA4427011 | B | Inhibition of full length V5 tagged human DAGLalpha expressed in African green monkey COS7 cells using sn-1-stearoyl-[14C]-arachidonoyl-glycerol as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by radiometric natural substrate assay | Homo sapiens | 1 | ALA4425142 | cell-based format | Scientific Literature | |
6. | ALA4427012 | B | Inhibition of full length 3' FLAG-tagged mouse DAGLbeta expressed in African green monkey COS7 cells using sn-1-stearoyl-[14C]-arachidonoyl-glycerol as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by radiometric natural substrate assay | Mus musculus | 1 | ALA4425142 | cell-based format | Scientific Literature | |
7. | ALA4427013 | B | Inhibition mouse DAGLbeta expressed in HEK293T cell membrane fractions using DAG as substrate preincubated for 30 mins followed by ABP HT01 probe addition measured after 30 mins in presence of probe by gel-based ABPP competition assay | Mus musculus | 5 | ALA4425142 | cell-based format | Scientific Literature | |
8. | ALA4427014 | B | Inhibition human DAGLalpha expressed in HEK293T cell membrane fractions using DAG as substrate preincubated for 30 mins followed by ABP HT01 probe addition measured after 30 mins in presence of probe by gel-based ABPP competition assay | Homo sapiens | 5 | ALA4425142 | cell-based format | Scientific Literature |