# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4359657 | Binding | Inhibition of wild type full length human JAK2 (1 to 1132 residues) autophosphorylation expressed in HEK293T cells at >10 uM incubated in buffer with compound for 1 hr followed by treatment with enzyme and [gamma32P]-ATP for 15 mins by immunoprecipitation assay based autoradiography | Homo sapiens | 1 | ALA4354882 | cell-based format | Scientific Literature | |
2. | ALA4359659 | Unassigned | Selectivity index, ratio of Kd for wild type human N-terminal TEV cleavage site-fused-His6-tagged JAK2 JH1 domain (840 to 1132 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay to Kd for wild type human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain (536 to 812 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay | Homo sapiens | 1 | ALA4354882 | cell-based format | Scientific Literature | |
3. | ALA4359660 | Binding | Binding affinity to wild type human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain (536 to 812 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay | Homo sapiens | 15 | ALA4354882 | cell-based format | Scientific Literature | |
4. | ALA4359661 | Binding | Binding affinity to human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain V617F mutant (536 to 812 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay | Homo sapiens | 4 | ALA4354882 | cell-based format | Scientific Literature | |
5. | ALA4359662 | ADME | Binding affinity to wild type human N-terminal TEV cleavage site-fused/His6-tagged JAK2 JH1 domain (840 to 1132 residues) expressed in baculovirus infected sf9 insect cells measured for1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay | Homo sapiens | 4 | ALA4354882 | cell-based format | Scientific Literature | |
6. | ALA4359663 | Binding | Binding affinity to wild type human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain (536 to 812 residues) expressed in baculovirus infected sf9 insect cells by microscale thermophoresis assay | Homo sapiens | 4 | ALA4354882 | cell-based format | Scientific Literature | |
7. | ALA4359664 | Binding | Binding affinity to human C-terminal thrombin cleavage site-fused/6xHis-tagged JAK2 JH2 pseudokinase domain W777A/F974AH/V617F triple mutant (536 to 812 residues) expressed in baculovirus infected sf9 insect cells by microscale thermophoresis assay | Homo sapiens | 4 | ALA4354882 | cell-based format | Scientific Literature | |
8. | ALA4359665 | Unassigned | Selectivity index, Ratio of Kd for wild type human N-terminal TEV cleavage site-fused-His6-tagged JAK2 JH1 domain (840 to 1132 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay to Kd for binding affinity to human C-terminal thrombin cleavage site-fused/ 6xHis-tagged JAK2 JH2 pseudokinase domain V617F mutant (536 to 812 residues) expressed in baculovirus infected sf9 insect cells measured for 1 hr in presence of fluorescein conjugate tracer by competitive fluorescence polarization assay | Homo sapiens | 1 | ALA4354882 | cell-based format | Scientific Literature | |
9. | ALA4359666 | Binding | Inhibition of wild type full length human JAK2 (1 to 1132 residues) autophosphorylation expressed in HEK293T cells incubated in buffer with compound for 1 hr followed by treatment with enzyme and [gamma32P]-ATP for 15 mins by immunoprecipitation assay based autoradiography | Homo sapiens | 2 | ALA4354882 | cell-based format | Scientific Literature | |
10. | ALA4359667 | Binding | Inhibition of full length human C-terminal FLAG-tagged JAK2 V617F mutant (1 to 1132 residues) autophosphorylation expressed in HEK293T cells incubated in buffer with compound for 1 hr followed by treatment with enzyme and [gamma32P]-ATP for 15 mins by immunoprecipitation assay based autoradiography | Homo sapiens | 2 | ALA4354882 | cell-based format | Scientific Literature | |
11. | ALA4359670 | Binding | Inhibition of full length human C-terminal FLAG-tagged JAK2 V617F mutant (1 to 1132 residues) autophosphorylation expressed in HEK293T cells at <25 uM incubated in buffer with compound for 1 hr followed by treatment with enzyme and [gamma32P]-ATP for 15 mins by immunoprecipitation assay based autoradiography | Homo sapiens | 1 | ALA4354882 | cell-based format | Scientific Literature |