# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4356587 | Binding | Inhibition of recombinant C-terminal His6x-tagged human Mcl-1 (171 to 327 residues) interaction with biotinylated human Bim (51 to 76 residues) incubated for 60 mins in presence of 5 nM biotin-Bim by HTRF assay | Homo sapiens | 21 | ALA4354823 | single protein format | Scientific Literature | |
2. | ALA5096341 | Binding | Inhibition of recombinant His6-tagged human MCL1 (171 to 327 residues) expressed in Escherichia coli and biotinylated Bim BH3 peptide interaction using Biotin-DMRPEIWIAQELRRIGDEFNAYYARR as substrate incubated for 60 mins by TR-FRET analysis | Homo sapiens | 12 | ALA5096144 | assay format | Scientific Literature | |
3. | ALA5096342 | Functional | Anticancer activity against human OPM-2 cells assessed as inhibition of cell viability incubated for 24 hrs in presence of 10% FBS by Cell-titer Glo assay | Homo sapiens | 12 | ALA5096144 | cell-based format | Scientific Literature |