# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4353688 | Binding | Inhibition of N-terminal His-tagged recombinant human KDM4A (1 to 359 residues) expressed in Escherichia coli assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay | Homo sapiens | 33 | ALA4350996 | assay format | Scientific Literature | |
2. | ALA4353689 | Binding | Inhibition of N-terminal GST-tagged recombinant human KDM4B (1 to 500 residues) expressed in baculovirus infected Sf9 insect cells assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay | Homo sapiens | 33 | ALA4350996 | cell-based format | Scientific Literature | |
3. | ALA4353690 | Binding | Inhibition of KDM5B (unknown origin) assessed as decrease in demethylation of substrate using peptide (H3(1-21)K4-Me3-GGKBiotin) as substrate and 2OG as cosubstrate pre-incubated for 15 mins before substrate addition followed by centrifugation for 15 secs and measured after 20 mins by Alphascreen assay | Homo sapiens | 33 | ALA4350996 | single protein format | Scientific Literature | |
4. | ALA4353691 | Binding | Inhibition of KDM5C (unknown origin) assessed as decrease in demethylation of substrate using peptide (H3(1-21)K4-Me3-GGKBiotin) as substrate and 2OG as cosubstrate pre-incubated for 15 mins before substrate addition followed by centrifugation for 15 secs and measured after 20 mins by Alphascreen assay | Homo sapiens | 33 | ALA4350996 | single protein format | Scientific Literature | |
5. | ALA4353732 | ADME | Apparent permeability of the compound in human Caco2 cells from apical to basolateral side at 10 uM after 2 hr by LC/MS/MS analysis | Homo sapiens | 33 | ALA4350996 | cell-based format | Scientific Literature |