| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4309946 | Binding | Binding affinity to human 15N-labeled STEP PTP domain (281 to 563 residues) expressed in Escherichia coli OD2 assessed as induction of chemical perturbation at 500 uM by 1H,15N TROSY NMR spectral analysis | Homo sapiens | 5 | ALA4308845 | assay format | Scientific Literature | |
| 2. | ALA4309948 | Binding | Binding affinity to human 15N-labeled STEP PTP domain (281 to 563 residues) expressed in Escherichia coli OD2 by 1H,15N TROSY NMR spectral analysis | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 3. | ALA4309949 | Binding | Binding affinity to human STEP PTP domain (281 to 563 residues) expressed in Escherichia coli BL21(DE3) by ITC | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 4. | ALA4309950 | Binding | Binding affinity to human PTP1B by 1H,15N TROSY NMR spectral analysis | Homo sapiens | 1 | ALA4308845 | single protein format | Scientific Literature | |
| 5. | ALA4309954 | Binding | Activation of human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) at 500 uM using biotin-pFyn peptide substrate after 60 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 6. | ALA4309955 | Binding | Activation of human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) at 100 uM using biotin-pFyn peptide substrate after 60 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 7. | ALA4309956 | Binding | Activation of human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) using biotin-pFyn peptide substrate after 60 mins by AlphaLISA assay | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 8. | ALA4309957 | Binding | Activation of human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) using pFyn peptide substrate after 60 mins by RapidFire MS/MS assay relative to control | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 9. | ALA4309958 | Binding | Activation of human STEP PTP domain (281 to 563 residues) expressed in Escherichia coli BL21(DE3) at 500 uM using biotin-pFyn peptide substrate after 60 mins by AlphaLISA assay relative to control | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 10. | ALA4309959 | Binding | Activation of human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) at 1000 uM preincubated for 10 mins followed by DiFMUP substrate addition and measured at 1 min time interval for 30 mins by fluorescence assay relative to control | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 11. | ALA4309960 | Binding | Activation of human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) at 300 uM preincubated for 10 mins followed by DiFMUP substrate addition and measured at 1 min time interval for 30 mins by fluorescence assay relative to control | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 12. | ALA4309961 | Binding | Activation of human PTP1B (1 to 405 residues) at 500 uM preincubated for 10 mins followed by EDT-pY-Y-RKG-amide substrate addition and measured after 1.5 hrs by AlphaLISA assay | Homo sapiens | 1 | ALA4308845 | single protein format | Scientific Literature | |
| 13. | ALA4309962 | Binding | Activation of human TCPTP (2 to 345 residues) at 500 uM using biotin-Ahx-EDT-pY-Y-RKG-amide as substrate measured after 2 hrs by AlphaLISA assay | Homo sapiens | 1 | ALA4308845 | single protein format | Scientific Literature | |
| 14. | ALA4309963 | Binding | Activation of human PTP1B (1 to 405 residues) at 500 uM preincubated for 10 mins followed by pFYN substrate addition and measured after 1.5 hrs by AlphaLISA assay | Homo sapiens | 1 | ALA4308845 | single protein format | Scientific Literature | |
| 15. | ALA4309964 | Binding | Activation of mouse STEP PTP domain (244 to 539 residues) expressed in Escherichia coli BL21(DE3) at 500 uM using biotin-pFyn peptide substrate after 60 mins by AlphaLISA assay | Mus musculus | 1 | ALA4308845 | assay format | Scientific Literature | |
| 16. | ALA4309965 | Binding | Activation of human STEP PTP domain (281 to 563 residues) expressed in Escherichia coli BL21(DE3) assessed as Km for substrate at 500 uM using pFyn peptide substrate after 60 mins by RapidFire MS/MS assay (Rvb = 60.8 +/- 7.9 uM) | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 17. | ALA4309966 | Binding | Activation of human STEP PTP domain (281 to 563 residues) expressed in Escherichia coli BL21(DE3) assessed as Kcat at 500 uM using pFyn peptide substrate after 60 mins by RapidFire MS/MS assay (Rvb = 0.024 +/- 0.002 /s) | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 18. | ALA4309967 | Binding | Activation of human STEP PTP domain (281 to 563 residues) expressed in Escherichia coli BL21(DE3) assessed as ratio of Kcat to Km at 500 uM using pFyn peptide substrate after 60 mins by RapidFire MS/MS assay (Rvb = 400 +/- 20 /M/s) | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature | |
| 19. | ALA4309968 | Binding | Binding affinity to human STEP46 (197 to 565 residues) expressed in Escherichia coli BL21(DE3) assessed as increase in melting temperature at 500 uM by SYPRO Orange dye based DSF | Homo sapiens | 1 | ALA4308845 | assay format | Scientific Literature |
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