| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4376261 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 1.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 26 | ALA4373727 | cell-based format | Scientific Literature | |
| 2. | ALA4376262 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 Rosetta (DE3) assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 4.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 2 | ALA4373727 | assay format | Scientific Literature | |
| 3. | ALA4376263 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 Rosetta (DE3) assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 15 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 2 | ALA4373727 | assay format | Scientific Literature | |
| 4. | ALA4376264 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
| 5. | ALA4376265 | B | Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Mycobacterium tuberculosis | 3 | ALA4373727 | assay format | Scientific Literature | |
| 6. | ALA4376266 | B | Binding affinity to full length Staphylococcus aureus tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Staphylococcus aureus | 3 | ALA4373727 | assay format | Scientific Literature | |
| 7. | ALA4376267 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as association rate constant by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
| 8. | ALA4376268 | B | Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) assessed as association rate constant by surface plasmon resonance assay | Mycobacterium tuberculosis | 3 | ALA4373727 | assay format | Scientific Literature | |
| 9. | ALA4376269 | B | Binding affinity to full length Staphylococcus aureus tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) assessed as association rate constant by surface plasmon resonance assay | Staphylococcus aureus | 3 | ALA4373727 | assay format | Scientific Literature | |
| 10. | ALA4376270 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as dissociation rate constant by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
| 11. | ALA4376271 | B | Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) assessed as dissociation rate constant by surface plasmon resonance assay | Mycobacterium tuberculosis | 3 | ALA4373727 | assay format | Scientific Literature | |
| 12. | ALA4376272 | B | Binding affinity to full length Staphylococcus aureus tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) assessed as dissociation rate constant by surface plasmon resonance assay | Staphylococcus aureus | 3 | ALA4373727 | assay format | Scientific Literature | |
| 13. | ALA4376273 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring change in melting temperature at 0.1 mM by SYPRO orange based thermal shift assay | Pseudomonas aeruginosa | 11 | ALA4373727 | cell-based format | Scientific Literature | |
| 14. | ALA4376275 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring melting temperature at 0.1 mM by SYPRO orange based thermal shift assay (Rvb = 50 degreeC) | Pseudomonas aeruginosa | 2 | ALA4373727 | cell-based format | Scientific Literature | |
| 15. | ALA4376277 | B | Selectivity ratio of Kd for full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) to Kd for Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells | 2 | ALA4373727 | cell-based format | Scientific Literature | ||
| 16. | ALA4376280 | B | Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization of protein by measuring melting temperature at 0.1 mM by SYPRO orange based thermal shift assay (Rvb = 54 degreeC) | Mycobacterium tuberculosis | 2 | ALA4373727 | assay format | Scientific Literature | |
| 17. | ALA4376283 | F | Antibacterial activity against methicillin-resistant Staphylococcus aureus ATCC 43300 at 100 uM incubated for 24 hrs | Staphylococcus aureus | 25 | ALA4373727 | organism-based format | Scientific Literature | |
| 18. | ALA4376284 | F | Antibacterial activity against Enterococcus faecalis ATCC 51299 at 100 uM incubated for 24 hrs | Enterococcus faecalis | 25 | ALA4373727 | organism-based format | Scientific Literature | |
| 19. | ALA4376285 | F | Antibacterial activity against Streptococcus pneumoniae ATCC 49619 at 100 uM incubated for 24 hrs | Streptococcus pneumoniae | 25 | ALA4373727 | organism-based format | Scientific Literature | |
| 20. | ALA4376286 | F | Antibacterial activity against Mycolicibacterium smegmatis MC2 155 at 100 uM incubated for 24 hrs | Mycolicibacterium smegmatis | 25 | ALA4373727 | organism-based format | Scientific Literature |
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