| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4392203 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells using GPKRIA as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 2. | ALA4392205 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells assessed as reduction in GPKRIA trimethylation at 50 nM preincubated for 10 mins in presence of SAM followed by GPKRIA addition and measured after 20 mins by MALDI TOF-MS analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA4392206 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells assessed as reduction in GPKRIA trimethylation at 100 nM preincubated for 10 mins in presence of SAM followed by GPKRIA addition and measured after 20 mins by MALDI TOF-MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA4392207 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells assessed as reduction in GPKRIA di-methylation at 100 nM preincubated for 10 mins in presence of SAM followed by GPKRIA addition and measured after 20 mins by MALDI TOF-MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4392208 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells preincubated for 10 mins in presence of SAM followed by GPKRIA addition and measured after 20 mins by MALDI TOF-MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4392209 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells assessed as residual enzyme activity at 3.3 uM using GPKRIA as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4392210 | B | Inhibition of recombinant human full-length His6-tagged NTMT1 (1 to 222 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL competent cells assessed as residual enzyme activity at 10 uM using GPKRIA as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4392211 | B | Inhibition of human His-tagged G9a (913 to 1193 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL assessed as enzyme residual activity at 3.3 uM using H3-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA4392212 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 3.3 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 10. | ALA4392213 | B | Inhibition of human His-tagged SETD7 (1 to 366 residues) expressed in Escherichia coli assessed as enzyme residual activity at 3.3 uM using H3-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4392214 | B | Inhibition of Trypanosoma brucei N-terminal His6-tagged PRMT7 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL assessed as enzyme residual activity at 3.3 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Trypanosoma brucei | 1 | assay format | Scientific Literature | ||
| 12. | ALA4392215 | B | Inhibition of human His-tagged NNMT (1 to 264 residues) expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 3.3 uM using 1-quinoline as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4392216 | B | Inhibition of SAHH (unknown origin) assessed as enzyme residual activity at 3.3 uM using SAH as substrate preincubated for 10 mins followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA4392217 | B | Inhibition of human His-tagged G9a (913 to 1193 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL assessed as enzyme residual activity at 10 uM using H3-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA4392218 | B | Inhibition of rat His6-tagged PRMT1 expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 10 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
| 16. | ALA4392219 | B | Inhibition of human His-tagged SETD7 (1 to 366 residues) expressed in Escherichia coli assessed as enzyme residual activity at 10 uM using H3-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA4392220 | B | Inhibition of Trypanosoma brucei N-terminal His6-tagged PRMT7 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL assessed as enzyme residual activity at 10 uM using H4-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Trypanosoma brucei | 1 | assay format | Scientific Literature | ||
| 18. | ALA4392221 | B | Inhibition of human His-tagged NNMT (1 to 264 residues) expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 10 uM using 1-quinoline as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 19. | ALA4392222 | B | Inhibition of SAHH (unknown origin) assessed as enzyme residual activity at 10 uM using SAH as substrate preincubated for 10 mins followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 20. | ALA4392223 | B | Inhibition of human His-tagged G9a (913 to 1193 residues) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL assessed as enzyme residual activity at 33 uM using H3-21 peptide as substrate preincubated for 10 mins in presence of SAM followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature |
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