| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4376141 | B | Inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 50 mins in presence of 70 nM [3H]-SAM by scintillation proximity assay | Homo sapiens | 7 | ALA4373722 | cell-based format | Scientific Literature | |
| 2. | ALA4376143 | B | Inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 120 mins in presence of 20 uM [3H]-SAM by scintillation proximity assay | Homo sapiens | 6 | ALA4373722 | cell-based format | Scientific Literature | |
| 3. | ALA4376146 | B | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in human MCF7 cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 4. | ALA4376147 | B | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in human MDA-MB-231 cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 5. | ALA4376148 | B | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in human HeLa cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 6. | ALA4376149 | B | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in HEK293 cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 7. | ALA4376150 | B | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in human CAL120 cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 8. | ALA4376151 | B | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in human U2OS cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 9. | ALA4376152 | A | Cytotoxicity against human MCF7 cells up to 10 uM measured after 5 days by IncuCyte zoom live cell analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 10. | ALA4376153 | A | Cytotoxicity against human U2OS cells up to 10 uM measured after 5 days by IncuCyte zoom live cell analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 11. | ALA4376154 | A | Cytotoxicity against human TERA2D cells up to 10 uM measured after 5 days by IncuCyte zoom live cell analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 12. | ALA4376155 | B | Competitive inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 50 mins in presence of varying levels of [3H]-SAM by scintillation proximity assay | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 13. | ALA4376156 | B | Mixed inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using varying levels of biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 50 mins in presence of 2 uM [3H]-SAM by scintillation proximity assay | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 14. | ALA4376157 | B | Inhibition of SUV420H1 (unknown origin) at 1 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA4373722 | single protein format | Scientific Literature | |
| 15. | ALA4376158 | B | Inhibition of SUV420H2 (unknown origin) at 1 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA4373722 | single protein format | Scientific Literature | |
| 16. | ALA4376159 | B | Selectivity ratio of IC50 for SUV420H1 (unknown origin) to IC50 for recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells at 40 uM using biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 50 mins in presence of 70 nM [3H]-SAM | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 17. | ALA4376160 | B | Inhibition of SUV420H1 in human MCF7 cells assessed as decrease in H4K20me3 level up to 50 uM measured after 2 days by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 18. | ALA4376161 | B | Inhibition of SUV420H2 in human MCF7 cells assessed as decrease in H4K20me3 level up to 50 uM measured after 2 days by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 19. | ALA4376162 | B | Selectivity ratio of IC50 for SUV420H1 in human MCF7 cells to IC50 for V5-tagged SMYD2 (unknown origin) transfected in human MCF7 cells co-transfected with FLAG-tagged p53 | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
| 20. | ALA4376163 | B | Selectivity ratio of IC50 for SUV420H2 in human MCF7 cells to IC50 for V5-tagged SMYD2 (unknown origin) transfected in human MCF7 cells co-transfected with FLAG-tagged p53 | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature |
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